2-Aminoethoxydiphenyl borate (2-APB) reduces respiratory burst, MMP-9 release and CD11 b expression, and increases L-selectin shedding in bovine neutrophils
2-Aminoethoxydiphenyl borate (2-APB) reduces respiratory burst, MMP-9 release and CD11 b expression, and increases L-selectin shedding in bovine neutrophils
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DOI：
10.1016/j.rvsc.2010.10.005
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发表时间：
2012-02-01
影响因子：
2.4
通讯作者：
Burgos, R. A.
中科院分区：
文献类型：
--
作者：
Conejeros, I.;Jara, E.;Burgos, R. A.
本研究描述了2-氨基乙氧基二苯基硼酸酯（2-APB），一种假定的钙库操纵的钙（Ca 2+）进入（SOCE）抑制剂，对活性氧（ROS）的产生，基质金属蛋白酶9（MMP-9）的释放，CD 11b和L-选择素（CD 62 L）的表达，血小板活化因子（PAF）刺激的牛中性粒细胞的大小变化和凋亡的影响。结果表明，>= 1 μ M的2-APB能显著降低PAF诱导的ROS产生，而50和100 μ M的2-APB能显著降低PAF诱导的MMP-9释放。此外，浓度>= 10 μ M的2-APB降低CD 11b表达和增加L-选择素脱落。PAF引起中性粒细胞大小改变，这种作用被2-APB抑制。从这项工作中可以得出结论，在抑制SOCE反应的浓度下，2-APB能够抑制ROS和MMP-9的释放和CD 11b的表达，并增加L-选择素的脱落，这表明SOCE中涉及的Ca 2+通道是开发牛新型抗炎药物的潜在靶点。(C)2010爱思唯尔有限公司版权所有。
This study describes the effect of 2-aminoethoxydiphenyl borate (2-APB), a putative store-operated calcium (Ca2+) entry (SOCE) inhibitor, on reactive oxygen species (ROS) production, matrix metalloproteinase 9 (MMP-9) release, CD11b and L-selectin (CD62L) expression, size changes and apoptosis in bovine neutrophils stimulated with platelet-activating factor (PAF). It was observed that doses >= 1 mu M 2-APB significantly reduced ROS production, whereas 50 and 100 mu M 2-APB reduced MMP-9 release induced by PAF. Moreover, concentrations >= 10 mu M 2-APB reduced CD11b expression and increased L-selectin shedding. PAF induced size changes in neutrophils, and this effect was inhibited by 2-APB. From this work it is possible to conclude that 2-APB at concentrations that inhibit SOCE responses was able to inhibit ROS and MMP-9 release and CD11b expression, and increase L-selectin shedding, suggesting that the Ca2+ channel involved in SOCE is a potential target for the development of new anti-inflammatory drugs in cattle. (C) 2010 Elsevier Ltd. All rights reserved.