Comparison of the effects of melatonin and genistein on radiation-induced nephrotoxicity: Results of an experimental study
Comparison of the effects of melatonin and genistein on radiation-induced nephrotoxicity: Results of an experimental study
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DOI：
10.3892/br.2015.547
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发表时间：
2016-01-01
影响因子：
2.3
通讯作者：
Yoney, Adnan
中科院分区：
文献类型：
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作者：
Canyilmaz, Emine;Uslu, Gonca Hanedan;Yoney, Adnan
本研究的目的是比较褪黑激素和金雀异黄素对辐射引起的肾毒性（RIN）的影响。总共 70 只瑞士白化小鼠被分为 7 组。定义五个对照组，分别为假放疗（C，G1）、仅放疗（RT，G2）、褪黑激素（M，G3）、金雀异黄素（G，G4）和聚乙二醇-400（G5）。联合治疗组为RT加褪黑激素组（RT+M，G6）和RT加染料木黄酮组（RT+G，G7）。使用钴 60 远程治疗机（80 厘米固定源到表面距离，2.5 厘米深度）进行照射。在单次照射前30分钟施用褪黑素(100mg/kg，腹腔注射)，而金雀异黄素在单次照射前1天施用(200mg/kg，皮下注射)。照射后6个月处死所有小鼠。作为终点，通过肾脏组织切片的图像分析来量化每只小鼠的肾小管萎缩程度。还测量了每只动物的组织丙二醛（MDA）水平。在小鼠肾脏的组织病理学检查中，有统计学上显着的减少（P
The aim of the present study was to compare the effects of melatonin and genistein on radiation-induced nephrotoxicity (RIN). A total of 70 Swiss Albino mice were divided into 7 groups. Five control groups were defined, which were sham irradiation (C, G1), radiation therapy only (RT, G2), melatonin (M, G3), genistein (G, G4) and polyethylene glycol-400 (G5), respectively. The co-treatment groups were the RT plus melatonin (RT+M, G6) and RT plus genistein (RT+G, G7) groups. Irradiation was applied using a cobalt-60 teletherapy machine (80-cm fixed source-to-surface distance, 2.5-cm depth). Melatonin was administered (100 mg/kg, intraperitoneal injection) 30 min before the single dose of irradiation, whereas genistein was administered (200 mg/kg, subcutaneous injection) 1 day before the single dose of irradiation. All the mice were sacrificed 6 months after irradiation. As an end point, the extent of renal tubular atrophy for each mouse was quantified with image analysis of histological sections of the kidney. Tissue malondialdehyde (MDA) levels were also measured in each animal. In the histopathological examination of the mouse kidneys, there was a statistically significant reduction (P