Fc (IgG) receptors on rat basophilic leukemia cells.
Fc (IgG) receptors on rat basophilic leukemia cells.
复制标题
大鼠嗜碱性白血病细胞上的 Fc (IgG) 受体。
DOI：
10.4049/jimmunol.126.1.138
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发表时间：
1981
影响因子：
4.4
通讯作者：
R. Siraganian
中科院分区：
文献类型：
--
作者：
D. Segal;S. Sharrow;J. F. Jones;R. Siraganian
用荧光和放射性方法检测大鼠嗜碱性白血病细胞IgG和IgE受体（RBL-IV的2H3亚系）对IgE和IgG的结合能力。2H3细胞的IgG受体大约是IgE受体的三分之二。IgE受体以高亲和力结合单体IgE，且高浓度的单体或寡聚IgG不抑制IgE的结合。相反，IgG受体结合（兔和大鼠）IgG和（大鼠）IgE。IgG受体对单体IgG和IgE的亲和力远低于IgE受体对其配体的亲和力；放射性标记的IgG或IgE与IgG受体的结合只能用交联低聚物检测。尽管这两种受体对胰蛋白酶消化都比IgE受体敏感。双激光流微荧光研究显示，在2H3细胞中，每个细胞中IgG和IgE受体的数量差异较大，且两种受体的分布相互独立。2H3细胞通过ige介导的系统释放组胺，但交联的IgG，即使在饱和量，也不会引起组胺释放，也不会对ige诱导的释放产生任何影响。此外，2H3细胞不会介导igg包被鸡红细胞的ADCC。
Receptors for IgG and IgE on rat basophilic leukemia cells (the 2H3 subline of RBL-IV) were detected by their ability bind IgE and IgG in fluorescence or radioactive assays. The 2H3 cells have approximately two-thirds as many receptors for IgG as for IgE. The IgE receptors bind monomeric IgE with high affinity and IgE binding is uninhibited by high concentrations of monomeric or oligomeric IgG. In contrast, the IgG receptors bind both (rabbit and rat) IgG and (rat) IgE. The affinity of IgG receptors for monomeric IgG and IgE is much lower than that of the IgE receptor for its ligand; binding of radiolabeled IgG or IgE to IgG receptors can be detected only with cross-linked oligomers. Although both receptors are sensitive to tryptic digestion than IgE receptors. Dual laser flow microfluorometric studies revealed that the numbers of IgG and IgE receptors per cell varied considerably among 2H3 cells and that the distributions of the 2 types of receptors were independent of each other. 2H3 cells released histamine through an IgE-mediated system, but cross-linked IgG, even in saturating amounts, did not elicit histamine release, nor have any effect upon IgE-induced release. Moreover, 2H3 cells would not mediate ADCC of IgG-coated chicken red blood cells.