Genome-Wide Analyses of Amphioxus MicroRNAs Reveal an Immune Regulation via miR-92d Targeting C3
Genome-Wide Analyses of Amphioxus MicroRNAs Reveal an Immune Regulation via miR-92d Targeting C3
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DOI：
10.4049/jimmunol.1200801
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发表时间：
2013-02
期刊：
影响因子：
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通讯作者：
Rirong Yang;Tingting Zheng;Xingsheng Cai;Yingcai Yu;Cuiling Yu;Lei Guo;Shengfeng Huang;Wei Zhu
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文献类型：
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作者：
Rirong Yang;Tingting Zheng;Xingsheng Cai;Yingcai Yu;Cuiling Yu;Lei Guo;Shengfeng Huang;Wei Zhu
近年来，文昌鱼已成为研究脊椎动物免疫起源和进化的模型。然而，关于microRNAs(MiRNAs)如何参与文昌鱼的免疫防御，人们知之甚少。在这篇文章中，我们系统地研究了文昌鱼miRNAs在细菌感染的急性期反应中的作用；miR-92d被发现在这个基础脊索酸中调节补体途径。我们通过生物信息学分析确定了文昌鱼基因组中所有155个可能的miRNAs，其中包括57个新发现的miRNAs(称为bbe-miRNAs)，并表征了miRNA的表达模式。在鳗弧菌和金黄色葡萄球菌的共同攻击下，4个miRNAs(bbe-miR-7、bbe-miR-4868a、bbe-miR-2065和bbe-miR-34b)表达上调，bbe-miR-92d表达下调。我们进一步利用混合聚合酶链式反应方法预测了miRNA靶点，并鉴定了免疫相关miRNA的mRNA靶点。我们认为miR-92d通过靶向C3来调节补体途径，从而控制对细菌感染的急性免疫反应。这项研究为miRNAs在基础脊索动物急性时相反应中的复杂免疫调节提供了证据。
Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we present a systematic study of amphioxus miRNAs in the acute-phase response to bacterial infection; miR-92d was found to regulate the complement pathway in this basal chordate. We identified all 155 possible miRNAs present in the amphioxus Branchiostoma belcheri genome by bioinformatics analyses, including 57 newly identified miRNAs (called bbe-miRNAs), and characterized the miRNA expression pattern. Four miRNAs (bbe-miR-7, bbe-miR-4868a, bbe-miR-2065, and bbe-miR-34b) were upregulated and bbe-miR-92d was downregulated under the challenge of both Vibrio anguillarum and Staphylococcus aureus bacteria. We further predicted miRNA targets and identified mRNA targets of immune-related miRNA using the hybrid PCR method. We propose that miR-92d regulates the complement pathway through targeting C3 for controlling the acute immune response to bacterial infections. This study provides evidence for the complex immune regulation of miRNAs in the acute-phase response in basal chordates.