Effect of a single bout of exercise on clock gene expression in human leukocyte
Effect of a single bout of exercise on clock gene expression in human leukocyte
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单次运动对人类白细胞时钟基因表达的影响
DOI：
10.1152/japplphysiol.00891.2019
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发表时间：
2020
影响因子：
3.3
通讯作者：
Tokuyama Kumpei
中科院分区：
文献类型：
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作者：
Tanaka Yoshiaki;Ogata Hitomi;Kayaba Momoko;Ando Akira;Park Insung;Yajima Katsuhiko;Araki Akihiro;Suzuki Chihiro;Osumi Haruka;Zhang Simeng;Ishihara Asuka;Takahashi Keigo;Shoda Junichi;Nabekura Yoshiharu;Satoh Makoto;Tokuyama Kumpei
哺乳动物具有昼夜节律钟，它由位于视交叉上核的中央钟和位于外周组织的外周钟组成。运动对外周时钟相位的影响在啮齿动物中有报道，但在人类中没有报道。连续采样是必要的，以评估外周时钟基因表达的昼夜节律的相位。白细胞中基因的表达被认为是“多器官转录组的一个可及窗口”。本研究旨在检测运动是否影响人类白细胞中时钟基因表达的水平和时相。11名年轻人参加了三项试验，其中他们在0700（早晨锻炼）或1600（下午锻炼）或不锻炼（对照）开始进行了一次60%V ~ o2 max的运动1小时。在0600、0900、1200、1500、1800、2100和2300以及第二天早上0600收集血液样品，以评估白细胞中时钟基因表达的昼夜变化。上午和下午运动后，大脑和肌肉ARNT样蛋白1（Bmal 1）表达水平增加，而隐花色素1（Cry 1）表达水平在上午运动后增加。与对照组相比，晨间运动组Bmal 1表达高峰期提前，午后运动组Bmal 1表达高峰期延迟。Cry 1表达高峰期在晨间运动试验中提前，但不受下午运动的影响。昼夜运动输出周期kaput（时钟），周期1-3（Per 1 -3），和Cry 2表达水平和这些顶相不受运动的影响。目前的研究结果表明，一个潜在的作用，一个回合的运动，以修改外周时钟在human.NEW & NOTEWORTHThe本研究表明，一个回合的运动影响外周时钟基因在人类白细胞的表达和运动的效果取决于何时进行。上午和下午运动后，脑和肌肉ARNT样蛋白1（Bmal 1）表达增加，隐花色素1（Cry 1）表达也增加。运动对Bmal 1峰时相的影响与运动时间有关，早晨运动后峰时相提前，下午运动后峰时相延迟。
Mammals have circadian clocks, which consist of the central clock in the suprachiasmatic nucleus and the peripheral clocks in the peripheral tissues. The effect of exercise on phase of peripheral clocks have been reported in rodents but not in humans. Continuous sampling is necessary to assess the phase of the circadian rhythm of peripheral clock gene expressions. It has been assumed that the expression of the genes in leukocyte may be “an accessible window to the multiorgan transcriptome.” The present study aimed to examine whether exercise affects the level and phase of clock gene expression in human leukocytes. Eleven young men participated in three trials, in which they performed a single bout of exercise at 60% V̇o2maxfor 1 h beginning either at 0700 (morning exercise) or 1600 (afternoon exercise) or no exercise (control). Blood samples were collected at 0600, 0900, 1200, 1500, 1800, 2100, and 2300 and at 0600 the next morning, to assess diurnal changes of clock gene expression in leukocytes. Brain and muscle ARNT-like protein 1 (Bmal1) expression level increased after morning and afternoon exercise, andCryptochrome 1(Cry1) expression level increased after morning exercise. Compared with control trial, acrophase ofBmal1expression tended to be earlier in morning exercise trial and later in afternoon exercise trial. Acrophase ofCry1expression was earlier in morning exercise trial but not affected by afternoon exercise. Circadian locomotor output cycles kaput (Clock),Period 1–3(Per1–3), andCry2expression levels and those acrophases were not affected by exercise. The present results suggest a potential role of a single bout of exercise to modify peripheral clocks in humans.NEW & NOTEWORTHYThe present study showed that a single bout of exercise affected peripheral clock gene expression in human leukocytes and the effect of exercise depended on when it was performed. Brain and muscle ARNT-like protein 1 (Bmal1) expression was increased after exercises performed in the morning and afternoon.Cryptochrome 1(Cry1) expression was also increased after the morning exercise. The effect of exercise on acrophase ofBmal1depended on the time of the exercise: advanced after morning exercise and delayed after afternoon exercise.