Luminescent Iridium(III) Complex Labeled DNA for Graphene Oxide-Based Biosensors
Luminescent Iridium(III) Complex Labeled DNA for Graphene Oxide-Based Biosensors
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用于氧化石墨烯生物传感器的发光铱 (III) 复合物标记 DNA
DOI：
10.1021/acs.analchem.5b04324
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发表时间：
2016
影响因子：
7.4
通讯作者：
Jian Liu
中科院分区：
文献类型：
--
作者：
Qingcheng Zhao;Yuyang Zhou;Yingying Li;Wei Gu;Qi Zhang;Jian Liu
利用高光稳定性的铱（III）配合物作为新的发光探针用于生物技术和生命科学的兴趣越来越大。本文合成了含羧基的铱（III）配合物，用N-羟基丁二酰亚胺活化后，标记到单链DNA（ssDNA）的氨基末端。Ir-ssDNA探针进一步与氧化石墨烯（GO）纳米片组合以开发用于靶ssDNA检测的基于GO的生物传感器。研究了GO的猝灭效率、铱配合物的光稳定性和GO-Ir-ssDNA生物传感器的光稳定性。基于GO对铱（III）络合物的高发光猝灭效率，GO-Ir-ssDNA生物传感器表现出最小的背景信号，而当Ir-ssDNA从GO纳米片解吸并与特定靶形成双螺旋时观察到强发射，导致高的信号背景比。此外，发现铱（III）配合物和GO-Ir-ssDNA生物传感器的发光强度分别是传统的羧基荧光素（FAM）染料和GO-FAM-ssDNA生物传感器的15倍和3倍。我们的研究表明，灵敏和选择性的Ir-ssDNA探针是适合于开发高光稳定的GO为基础的检测平台，显示出应用前景超出OLED（有机发光二极管）领域。
There has been growing interest in utilizing highly photostable iridium(III) complexes as new luminescent probes for biotechnology and life science. Herein, iridium(III) complex with carboxyl group was synthesized and activated withN-hydroxysuccinimide, followed by tagging to the amino terminate of single-stranded DNA (ssDNA). The Ir-ssDNA probe was further combined with graphene oxide (GO) nanosheets to develop a GO-based biosensor for target ssDNA detection. The quenching efficiency of GO, and the photostability of iridium(III) complex and GO-Ir-ssDNA biosensor, were also investigated. On the basis of the high luminescence quenching efficiency of GO toward iridium(III) complex, the GO-Ir-ssDNA biosensor exhibited minimal background signals, while strong emission was observed when Ir-ssDNA desorbed from GO nanosheets and formed a double helix with the specific target, leading to a high signal-to-background ratio. Moreover, it was found that luminescent intensities of iridium(III) complex and GO-Ir-ssDNA biosensor were around 15 and 3 times higher than those of the traditional carboxyl fluorescein (FAM) dye and the GO-FAM-ssDNA biosensor after UV irradiation, respectively. Our study suggested the sensitive and selective Ir-ssDNA probe was suitable for the development of highly photostable GO-based detection platforms, showing promise for application beyond the OLED (organic light emitting diode) area.