Fluorescence labeling of DNA based on photochemical ligation
Fluorescence labeling of DNA based on photochemical ligation
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DOI：
10.1016/j.stam.2005.12.007
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发表时间：
2006-04-01
影响因子：
5.5
通讯作者：
Fujimoto, Kenzo
中科院分区：
文献类型：
--
作者：
Ami, Takehiro;Fujimoto, Kenzo
寡核苷酸的荧光标记在用于区分和检测核酸序列的方法的开发方面已经吸引了人们的兴趣。而光化学连接法具有不需要额外试剂的优点。Fujimoto et at.报道了使用5-羧基乙烯基-脱氧尿苷（U-CV）的模板定向DNA光连接。本文报道了荧光报告类似物Cy 5连接的U-CV-ODN的合成及其光交联性能。(c)2006 NIMS和Elsevier Ltd.保留所有权利。
Fluorescent labeling of oligonucleotides has been attracting interest in connection with the development of methods for distinguishing and detecting nucleic acids sequences. And photochemical ligation has the merit of the avoiding the need for additional reagents. Fujimoto et at. reported template directed DNA photoligation using 5-carboxyvinyl-deoxyuridine (U-CV). Here, we describe the synthesis and photocross I inking ability of fluorescent reporter analogue (Cy5) tethered U-CV-containing ODN. (c) 2006 NIMS and Elsevier Ltd. All rights reserved.