Fast analysis of relevant contaminants mixture in commercial shellfish
Fast analysis of relevant contaminants mixture in commercial shellfish
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DOI：
10.1016/j.talanta.2019.04.085
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发表时间：
2019-12-01
期刊：
影响因子：
6.1
通讯作者：
Barcelo, D.
中科院分区：
文献类型：
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作者：
Alvarez-Munoz, D.;Rambla-Alegre, M.;Barcelo, D.
目前面临的主要挑战之一是开发系统的方法来处理环境评估中的化学混合物问题。为此，开发了一种简单、快速且灵敏的方法来检测和定量软体动物中相关污染物的混合物。该方法基于 QuEChERS（快速、简单、廉价、有效、坚固和安全）和超高效液相色谱-高分辨率质谱 (UHPLC-HRMS)。它包括由农药、内分泌干扰物和药物形成的23种化合物的混合物（异丙甲草胺、西玛津、去乙基莠去津、莠去津、噻菌灵、二嗪农、马拉硫磷、苯达松、二氯苯甲醚、敌稗、啶虫脒、吡虫啉、咖啡因、双酚A、三氯生、三氯卡班、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、 1H-苯并三唑、磺胺甲恶唑、文拉法辛和卡马西平）。该方法在 4 种具有高商业价值的不同类型的贝类中进行了开发和验证，例如贻贝 (Mytilus galloprovincialis)、牡蛎 (Crassostrea gigas)、鸟蛤 (Cerastoderma edule) 和剃刀贝 (Solen marginatus)。每种软体动物类型（种内）中所有化合物的平均回收率范围为 96% 至 107%，显示所开发方法的良好性能。日内分析和日间分析的相对标准偏差低于 10%。所有目标物种和化合物的方法检测限和方法定量限均低于 10 ng/g 干重。最后，在 2017 年发生了一些死亡事件后，该方法应用于来自埃布罗三角洲（西班牙）的水产养殖样品、牡蛎和鸟蛤。在 C. edule 中检测到高水平的双酚 A，这可能解释了这种生物体遭受的死亡。 C. gigas 的异丙甲草胺、灭草松、啶虫脒和对羟基苯甲酸甲酯含量较低。
One of the major challenges currently faced is to develop systematic ways of addressing chemical mixtures in environmental assessment. With this purpose, a simple, rapid, and sensitive method for the detection and quantification of a mixture of relevant contaminants in molluscs has been developed. The method is based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) and Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry (UHPLC-HRMS). It includes a mixture of 23 compounds formed by pesticides, endocrine disruptors and pharmaceuticals (metolachlor, simazine, desethylatrazine, atrazine, thiabendazole, diazinon, malathion, bentazone, MCPA, propanil, acetamiprid, imidacloprid, caffeine, bisphenol A, triclosan, triclocarban, methylparaben, ethylparaben, propylparaben, 1H-benzotriazole, sulfamethoxazole, venlafaxine and carbamazepine). The method was developed and validated in 4 different types of shellfish of high commercial interest such as mussel (Mytilus galloprovincialis), oyster (Crassostrea gigas), cockle (Cerastoderma edule) and razor shell (Solen marginatus). The mean percentage of recoveries obtained for all the compounds in each mollusc type (infra-specie) ranged from 96% to 107% showing the good performance of the method developed. The relative standard deviation was under 10% for the intra-day and 17% inter-day analyses. Method detection limits and method quantification limits were below 10 ng/g dry weight for all the species and compounds targeted. Finally, the method was applied to aquaculture samples, oysters and cockles, from Ebro Delta (Spain), after some episodes of mortality occurred in 2017. A high level of bisphenol A was detected in C. edule which may explain the mortality suffered by this organism. C. gigas presented low levels of metolachlor, bentazone, acetamiprid, and methylparaben.