TRANSFER AND EXPRESSION OF THE CATABOLIC PLASMID PBRC60 IN WILD BACTERIAL RECIPIENTS IN A FRESH-WATER ECOSYSTEM
TRANSFER AND EXPRESSION OF THE CATABOLIC PLASMID PBRC60 IN WILD BACTERIAL RECIPIENTS IN A FRESH-WATER ECOSYSTEM
复制标题
DOI：
10.1128/aem.57.5.1546-1553.1991
复制
发表时间：
1991-05-01
影响因子：
4.4
通讯作者：
WYNDHAM, RC
中科院分区：
文献类型：
--
作者：
FULTHORPE, RR;WYNDHAM, RC
3-从投加不同浓度3-Cba的流通式围隔生态系统的沃茨和沉积物中分离出3-Cba降解细菌，并接种3-Cba降解产碱菌，菌株BR 60。 分离出与BR 60不同的3Cba降解菌。 它们携带pBRC 60，这是一种引入产碱杆菌属菌株BR 60的质粒，该菌株携带编码3Cba降解的转座因子（Tn 5271）。 分离株以不同的方式表达这些基因。 大多数pBRC 60受体是能动的，黄色，革兰氏阴性杆菌相关的第III组假单胞菌和BR 60的底物利用模式。 它们能够在毫摩尔和微摩尔浓度下完全降解3Cba。 荧光假单胞菌PR 24 B（pBRC 60）和假单胞菌属菌株PR 120（pBRC 60）这两个菌株与BR 60的亲缘关系更远，在酵母提取物存在下暴露于毫摩尔浓度的3Cba时，这两个菌株都产生氯儿茶酚。 这些物种在液体3Cba基本培养基中生长不良，但在连续培养中以微摩尔水平的化学品给药时可降解3Cba。 实验室交配证实，pBRC 60可以从BR 60转移到变形菌的β和γ亚群中的物种，并且3Cba基因表达在物种之间是可变的。 选择压力作用于pBRC 60受体进行了讨论。
3-Chlorobenzoate (3Cba)-degrading bacteria were isolated from the waters and sediments of flowthrough mesocosms dosed with various concentrations of 3Cba and inoculated with a 3Cba-degrading Alcaligenes sp., strain BR60. Bacteria capable of 3Cba degradation which were distinct from BR60 were isolated. They carried pBRC60, a plasmid introduced with Alcaligenes sp. strain BR60 that carries a transposable element (Tn5271) encoding 3Cba degradation. The isolates expressed these genes in different ways. The majority of pBRC60 recipients were motile, yellow-pigmented, gram-negative rods related to the group III pseudomonads and to BR60 by substrate utilization pattern. They were capable of complete 3Cba degradation at both millimolar and micromolar concentrations. Two isolates, Pseudomonas fluorescens PR24B(pBRC60) and Pseudomonas sp. strain PR120(pBRC60), are more distantly related to BR60 and both produced chlorocatechol when exposed to 3Cba at millimolar concentrations in the presence of yeast extract. These species showed poor growth in liquid 3Cba minimal medium but could degrade 3Cba in continuous cultures dosed with micromolar levels of the chemical. Laboratory matings confirm that pBRC60 can transfer from BR60 to species in both the beta and gamma subgroups of the proteobacteria and that 3Cba gene expression is variable between species. Selection pressures acting on pBRC60 recipients are discussed.