Expression of basement membrane type IV collagen chains during postnatal development in the murine cochlea
Expression of basement membrane type IV collagen chains during postnatal development in the murine cochlea
复制标题
DOI：
10.1016/0378-5955(96)00114-1
复制
发表时间：
1996-10-01
期刊：
影响因子：
2.8
通讯作者：
Samuelson, G
中科院分区：
文献类型：
--
作者：
Cosgrove, D;Kornak, JM;Samuelson, G
免疫荧光研究进行检查的时间和空间模式的表达不同的IV型胶原蛋白链在出生后耳蜗发育。在出生时，经典链（4A1和4A2）广泛表达，而新链（4A3，4A4和4A5）完全缺失。在4天龄时观察到新链的激活，强烈的、广泛分布的免疫染色表明耳蜗中的大多数细胞在这个发育阶段表达新链。从第8天到第14天，新链的发育失活导致全身免疫反应性的降低，伴随着螺旋利姆布斯、内沟、基底膜、以及从螺旋突起放射到螺旋韧带区域的染色纤维床，该区域对应于根部的位置细胞突起这种新链的强烈免疫染色模式持续到成年。经典链在这些相同的解剖区域中仅短暂表达（从第6天至第10天），之后逐渐发育失活导致成人表达模式，其中经典胶原蛋白链主要存在于神经束膜、螺旋神经节细胞体周围的膜以及螺旋韧带和血管纹的血管基底膜中。小鼠耳蜗中IV型胶原蛋白链表达的复杂发育模式与小鼠肾脏中观察到的相似，后者是Alport综合征基底膜病理的另一个主要部位。
An immunofluorescence study was performed to examine the temporal and spatial patterns of expression for the different type IV collagen chains during postnatal cochlear development. At birth, the classical chains (4A1 and 4A2) were widely expressed, while the novel chains (4A3, 4A4, and 4A5) were completely absent. Activation of the novel chains was observed at 4 days of age, with intense, widely distributed immunostaining suggesting that most of the cells in the cochlea express the novel chains at this developmental stage. From day 8 through day 14, developmental inactivation of the novel chains results in a reduction of generalized immunoreactivity with a concomitant elevation of specific staining in the membranous structures bounding the interdental cells of the spiral limbus, the inner sulcus, the basilar membrane, and in a fibrous bed of staining radiating from the spiral prominence into the region of the spiral ligament which corresponds to the location of the root cell processes. This pattern of intense immunostaining for the novel chains persists through adulthood. The classical chains are expressed in these same anatomical regions only transiently (from day 6 to day 10), after which a gradual developmental inactivation leads to the adult expression pattern where classical collagen chains are found primarily in the perineurium, in the membranes surrounding the spiral ganglion cell bodies, and in the vascular basement membranes of the spiral ligament and the stria vascularis. The complex developmental pattern of expression for the type IV collagen chains in the murine cochlea is similar to that observed in the murine kidney, which is the other major site for basement membrane pathology in Alport syndrome.