Predicting death by the loss of intestinal function
Predicting death by the loss of intestinal function
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DOI：
10.1371/journal.pone.0230970
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发表时间：
2020-04-14
期刊：
影响因子：
3.7
通讯作者：
Mueller, Laurence D.
中科院分区：
文献类型：
--
作者：
Bitner, Kathreen;Shahrestani, Parvin;Mueller, Laurence D.
预测一个人何时死亡的能力对许多衰老研究问题都非常有用。一种预测黑腹果蝇（Drosophila melanogaster）这种模式生物死亡的技术已经被提出，该技术依赖于果蝇肠道系统渗透性的增加，使饮食中的染料在死亡前不久渗入果蝇的身体。在这项研究中，我们试图通过使用不同种群和不同染料的大型队列研究来验证这一说法。我们发现只有大约50%的个体在死亡前显示出可见的染料分布。这一数字与所使用的染料相差不大。大多数在死前变成蓝色的苍蝇都是在死后24小时内变成蓝色的。染料对果蝇的平均寿命也有可测量的影响。这些结果往往会限制该方法的效用，这取决于该方法所针对的应用程序。
The ability to predict when an individual will die can be extremely useful for many research problems in aging. A technique for predicting death in the model organism, Drosophila melanogaster, has been proposed which relies on an increase in the permeability of the fly intestinal system, allowing dyes from the diet to permeate the body of the fly shortly before death. In this study we sought to verify this claim in a large cohort study using different populations of D. melanogaster and different dyes. We found that only about 50% of the individuals showed a visible distribution of dye before death. This number did not vary substantially with the dye used. Most flies that did turn a blue color before death did so within 24 hours of death. There was also a measurable effect of the dye on the fly mean longevity. These results would tend to limit the utility of this method depending on the application the method was intended for.