EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila
EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila
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DOI：
10.1073/pnas.012555499
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发表时间：
2002-01-08
影响因子：
11.1
通讯作者：
Osborne, HB
中科院分区：
文献类型：
--
作者：
Ezzeddine, N;Paillard, L;Osborne, HB
翻译控制是调节卵母细胞和卵子中基因表达的关键水平，因为许多mrna在卵发生过程中被合成和储存，以供卵母细胞成熟和胚胎发育的各个阶段使用。因此，理解这种转化控制的分子机制至关重要。另一个重要的问题是这些机制的进化守恒——换句话说，决定它们的普遍和特殊方面。我们在这里报告了一个翻译抑制机制的比较分析，该机制依赖于EDEN（胚胎死化元件）元件。这个小的顺式作用元件位于c-mos和Eg mrna的3'非翻译区，被证明参与死烯化过程。我们在此证明，在非洲爪蟾胚胎中，含有EDEN的mrna在翻译上受到抑制。接下来，利用转基因果蝇研究了EDEN基序对果蝇卵母细胞翻译的影响。我们发现该元件也会导致果蝇报告基因的翻译抑制，这表明依赖于eden的翻译抑制在爪蟾和果蝇之间在功能上是保守的。
Translational control is a key level in regulating gene expression in oocytes and eggs because many mRNAs are synthesized and stored during oogenesis for latter use at various stages of oocyte maturation and embryonic development. Understanding the molecular mechanisms that underlie this translational control is therefore crucial. Another important issue is the evolutionary conservation of these mechanisms-in other words the determination of their universal and specific aspects. We report here a comparative analysis of a translational repression mechanism that depends on the EDEN (embryo deadenylation element) element. This small cis-acting element, localized in the 3' untranslated region of c-mos and Eg mRNAs, was shown to be involved in a deadenylation process. We demonstrate here that in Xenopus embryos, mRNAs that contain an EDEN are translationally repressed. Next, transgenic flies were used to study the effect of the EDEN motif on translation in Drosophila oocytes. We show that this element also causes the translational repression of a reporter gene in Drosophila demonstrating that the EDEN-dependent translational repression is functionally conserved between Xenopus and Drosophila.