PCaP2 regulates nuclear positioning in growing Arabidopsis thaliana root hairs by modulating filamentous actin organization
PCaP2 regulates nuclear positioning in growing Arabidopsis thaliana root hairs by modulating filamentous actin organization
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PCaP2通过调节丝状肌动蛋白组织来调节拟南芥根毛生长中的核定位
DOI：
10.1007/s00299-015-1789-6
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发表时间：
2015
影响因子：
6.2
通讯作者：
Zhu Lei
中科院分区：
文献类型：
--
作者：
Zhang Yan;Kang Erfang;Yuan Ming;Fu Ying;Zhu Lei
关键信息PCaP2通过调节肌动蛋白丝，在根毛生长过程中维持细胞核与顶端相对固定的距离方面发挥着关键作用。摘要在根毛生长过程中，细胞核定位在与顶端相对固定的距离处。在拟南芥中，细胞核的位置主要取决于微丝（丝状肌动蛋白）的构型。然而，肌动蛋白动力学和核定位组织的调节机制在很大程度上尚不清楚。在本研究中，我们证明质膜相关的 Ca2+ 结合蛋白 2 (PCaP2) 在根毛生长过程中影响细胞核的位置。 PCaP2在pcap2和PCaP2过表达植物中的异常表达导致根毛顶端和亚顶端区域的肌动蛋白丝而不是微管的紊乱，从而导致根毛生长模式异常和细胞核错位。使用 PCaP2 突变蛋白进行的分析表明，肌动蛋白切断活性对于根毛中 PCaP2 的功能至关重要。我们证明 PCaP2 通过调节肌动蛋白丝在根毛生长中维持核位置方面发挥着关键作用。
Key messagePCaP2 plays a key role in maintaining the nucleus at a relatively fixed distance from the apex during root hair growth by modulating actin filaments.AbstractDuring root hair growth, the nucleus localizes at a relatively fixed distance from the apex. InArabidopsis thaliana, the position of the nucleus is mainly dependent on the configuration of microfilaments (filamentous actin). However, the mechanisms underlying the regulation of actin dynamics and organization for nuclear positioning are largely unknown. In the present study, we demonstrated that plasma membrane-associated Ca2+binding protein 2 (PCaP2) influences the position of the nucleus during root hair growth. Abnormal expression ofPCaP2inpcap2andPCaP2 over-expressionplants led to the disorganization of actin filaments, rather than microtubules, in the apex and sub-apical regions of root hairs, which resulted in aberrant root hair growth patterns and misplaced nuclei. Analyses using a PCaP2 mutant protein revealed that actin-severing activity is essential for the function of PCaP2 in root hairs. We demonstrated that PCaP2 plays a key role in maintaining nuclear position in growing root hairs by modulating actin filaments.