Predominant actions of cytosolic BSU1 and nuclear BIN2 regulate subcellular localization of BES1 in brassinosteroid signaling
Predominant actions of cytosolic BSU1 and nuclear BIN2 regulate subcellular localization of BES1 in brassinosteroid signaling
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DOI：
10.1007/s10059-010-0034-y
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发表时间：
2010-03-01
影响因子：
3.8
通讯作者：
Hwang, Ildoo
中科院分区：
文献类型：
--
作者：
Ryu, Hojin;Kim, Kangmin;Hwang, Ildoo
在拟南芥油菜素内酯（brassino类固醇）信号传导过程中，BSU1 （bri1 SUPPRESSOR1）磷酸酶和BIN2 （brassino类固醇INSENSITIVE2）激酶通过决定转录因子BZR1 （BRASSINAZOLE RESISTANT1）和BES1 （bri1 EMS SUPPRESSOR1）的磷酸化状态来调节信号强度。在这里，我们报道了BIN2和BSU1是核质调控因子，可以调节BES1的亚细胞定位，在细胞核和细胞质之间具有不同的活性。在我们的实验中，核BIN2比细胞质BIN2更有效地诱导磷酸化离子和BES1的核输出。细胞质BSU1介导BES1的去磷酸化和核易位的效率高于细胞核。BSU1对BRI1 （BR-INSENSITIVE 1）受体激酶的弱等位基因BRI1 -5的矮化表型的影响，在转基因植物中定位于细胞质比定位于细胞核更有效。综上所述，BES1在BR信号传导中有效的亚细胞定位可能需要细胞质BSU1和核BIN2的优势。
During brassinosteroid (BR)signaling in Arabidopsis, BSU1 (bri1 SUPPRESSOR1) phosphatase and BIN2 (BRASSINOSTEROID INSENSITIVE2) kinase regulate the signal intensity by determining the phosphorylation status of the transcription factors BZR1 (BRASSINAZOLE RESISTANT1) and BES1 (bri1 EMS SUPPRESSOR1). Here, we report hat BIN2 and BSU1 are nucleocy oplasmic regulators that modulate the subcellular localization of BES1, with differential activities between the nucleus and the cytosol.In our experiments, the nuclear BIN2 induced phosphoryla ion and nuclear expor of BES1 more efficiently than cytosolic BIN2.The cytoplasmic BSU1 mediated the dephosphorylation and nuclear translocation of BES1 more efficiently than the nuclear one. BSU1 compromised the dwarf phenotype of bri1-5, a weak allele of BRI1 (BR-INSENSITIVE 1) receptor kinase, more effectively when localized in the cytosol than in the nucleus in transgenic plants. In conclusion, the predominance of cytosolic BSU1 and nuclear BIN2 might be required for the efficient subcellular localization of BES1 in BR signaling.