ESCHERICHIA-COLI K-12 TOLF MUTANTS - ALTERATIONS IN PROTEIN-COMPOSITION OF OUTER MEMBRANE
ESCHERICHIA-COLI K-12 TOLF MUTANTS - ALTERATIONS IN PROTEIN-COMPOSITION OF OUTER MEMBRANE
复制标题
DOI：
10.1128/jb.130.2.781-786.1977
复制
发表时间：
1977-01-01
影响因子：
3.2
通讯作者：
FOULDS, J
中科院分区：
文献类型：
--
作者：
CHAI, TJ;FOULDS, J
外膜材料由3个独立分离的自发E. colitolF突变体中未检测到Ia蛋白。这种蛋白质的损失几乎完全由其他主要外膜蛋白Ib和II* 的增加所补偿。因此，在tol+和tolF菌株中，主要外膜蛋白占总细胞包膜蛋白的40%。当与来自tol+菌株的类似制剂相比时，未发现从tolF菌株制备的内膜蛋白水平的变化。通过使用噬菌体TuIb或噬菌体PA2从tolF菌株开始选择噬菌体抗性突变体。这些抗噬菌体的tolF菌株既不含蛋白Ia也不含蛋白Ib。这些菌株中导致蛋白Ib缺失的突变与tolF突变无关，位于E.大肠杆菌遗传图谱。
Outer membrane materials prepared from 3 independently isolated spontaneous E. coli tolF mutants contained no detectable protein Ia. The loss of this protein was nearly completely compensated for by an increase in other major outer membrane proteins, Ib and II*. Thus, the major outer membrane proteins accounted for 40% of the total cell envelope protein in both tol+ and tolF strains. No changes were found in the levels of inner membrane proteins prepared from tolF strains when compared with similar preparations from the tol+ strain. Phage-resistant mutants were selected starting with a tolF strain by using phage TuIb or phage PA2. These phage-resistant tolF strains contained neither protein Ia nor protein Ib. The mutation leading to the loss of protein Ib in these strains is independent of the tolF mutation and is located near malP on the E. coli genetic map.