DNA sequencing based on intrinsic molecular charges
DNA sequencing based on intrinsic molecular charges
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DOI：
10.1002/anie.200503154
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发表时间：
2006-01-01
影响因子：
16.6
通讯作者：
Miyahara, Y
中科院分区：
文献类型：
--
作者：
Sakata, T;Miyahara, Y
在完成人类基因组解码的基础上，基因功能分析在分子生物学、药物基因组学和临床研究等领域取得了显著进展。核苷酸变异分析对于构建疾病相关基因座的高分辨率图谱和临床诊断变得越来越重要。最常见的基因组变异形式是单核苷酸多态(SNP)，它是个性化药物中影响疾病易感性和耐药性的重要标记。尽管已经开发了许多SNP分析方法，[1-7]DNA测序技术在成本、简单性和吞吐量方面仍然需要改进，不仅要分析SNP，还要分析基因组变异，如插入/缺失和短串联重复。我们一直在研究一种新的方法，在小型化和阵列化的固态设备上直接、简单和高灵敏地检测非标记分子识别事件。[8]最近，几种类型的场效应设备被用于固体表面杂交事件的电化学检测。[9]就像DNA分子一样。
Gene functional analyses have proceeded remarkably in the fields of molecular biology, pharmacogenomics, and clinical research, on the basis of completion of the decoding of the human genome. The analysis of nucleotide variation has become increasingly important for the assembly of a highresolution map of disease-related loci and for clinical diagnostics. The most common form of genomic variation is single-nucleotide polymorphism (SNP), which is an important marker in personalized medicine that affects disease susceptibility and resistance. Although a number of methods for SNP analysis have been developed,[1–7] DNA sequencing techniques still need to be improved in terms of cost, simplicity, and throughput to analyze not only SNPs but also genomic variations, such as insertion/deletion and short tandem repeats.We have been investigating a new approach to the direct, simple, and highly sensitive detection of nonlabeled molecular recognition events on a miniaturized and arrayed solidstate device.[8] Recently, several types of field-effect devices have been used for the electrochemical detection of hybridization events on a solid surface.[9] As DNA molecules are