Expression of FcFT1, a FLOWERING LOCUS T-like gene, is regulated by light and associated with inflorescence differentiation in fig (Ficus carica L.).
Expression of FcFT1, a FLOWERING LOCUS T-like gene, is regulated by light and associated with inflorescence differentiation in fig (Ficus carica L.).
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DOI：
10.1186/1471-2229-13-216
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发表时间：
2013-12-16
影响因子：
5.3
通讯作者：
Nakahara T
中科院分区：
文献类型：
--
作者：
Ikegami H;Nogata H;Inoue Y;Himeno S;Yakushiji H;Hirata C;Hirashima K;Mori M;Awamura M;Nakahara T
由于成花诱导发生在许多植物中，当特定的环境条件得到满足时，大多数植物在每年的同一季节开花和结果。相比之下，在图中，花序（花芽、果实）分化发生的时间间隔对应于芽伸长期。因此，无花果树在其生殖生长特性上不同于许多物种。然而，迄今为止，无花果树成花诱导和坐果的非正统生理学背后的分子机制尚未阐明。我们从无花果中分离了一个类似FLOWERLOCUS T（FT）的基因，并研究了它的功能、特性和表达模式。分离的基因F.无花果FT（FcFT 1）与苹果MdFT 2在氨基酸水平上的相似性最高（93.1%）。我们对其上游区域（1，644 bp）进行了测序，并鉴定了许多光响应元件。FcFT 1主要在叶片中表达，并诱导烟草提前开花，表明FcFT 1是一个Fig FT的同源基因。实时荧光定量RT-PCR分析表明，FcFT 1 mRNA的表达仅发生在结果早期的下部节位叶片中。mRNA水平保持恒定的约5个月，从春季到秋季，几乎完全对应的花序分化季节。昼夜变化分析表明，FcFT 1 mRNA表达在相对长日照和短日照条件下均增加，而在连续黑暗条件下不增加。这些结果表明，FcFT 1的激活受光条件的调节，并可能有助于无花果的独特的坐果特性。
Because the floral induction occurs in many plants when specific environmental conditions are satisfied, most plants bloom and bear fruit during the same season each year. In fig, by contrast, the time interval during which inflorescence (flower bud, fruit) differentiation occurs corresponds to the shoot elongation period. Fig trees thus differ from many species in their reproductive growth characteristics. To date, however, the molecular mechanisms underlying this unorthodox physiology of floral induction and fruit setting in fig trees have not been elucidated. We isolated a FLOWERING LOCUS T (FT)-like gene from fig and examined its function, characteristics, and expression patterns. The isolated gene, F. carica FT (FcFT1), is single copy in fig and shows the highest similarity at the amino acid level (93.1%) to apple MdFT2. We sequenced its upstream region (1,644 bp) and identified many light-responsive elements. FcFT1 was mainly expressed in leaves and induced early flowering in transgenic tobacco, suggesting that FcFT1 is a fig FT ortholog. Real-time reverse-transcription PCR analysis revealed that FcFT1 mRNA expression occurred only in leaves at the lower nodes, the early fruit setting positions. mRNA levels remained a constant for approximately 5 months from spring to autumn, corresponding almost exactly to the inflorescence differentiation season. Diurnal variation analysis revealed that FcFT1 mRNA expression increased under relative long-day and short-day conditions, but not under continuous darkness. These results suggest that FcFT1 activation is regulated by light conditions and may contribute to fig’s unique fruit-setting characteristics.
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影响因子：
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作者：
Endo, M;Nakamura, S;Nagatani, A
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