CD23 REGULATES MONOCYTE ACTIVATION THROUGH A NOVEL INTERACTION WITH THE ADHESION MOLECULES CD11B-CD18 AND CD11C-CD18
CD23 REGULATES MONOCYTE ACTIVATION THROUGH A NOVEL INTERACTION WITH THE ADHESION MOLECULES CD11B-CD18 AND CD11C-CD18
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DOI：
10.1016/1074-7613(95)90164-7
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发表时间：
1995-07-01
期刊：
影响因子：
32.4
通讯作者：
BONNEFOY, JY
中科院分区：
文献类型：
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作者：
LECOANETHENCHOZ, S;GAUCHAT, JF;BONNEFOY, JY
CD 23在多种造血细胞上表达，并在体外显示多效性活性。我们报告，除了CD 21和IgE，CD 23特异性地与CD 11b和CD 11 c相互作用，β 2整合素粘附分子复合物CD 11b-CD 18和CD 11 c-CD 18的α链，在单核细胞上。荧光脂质体包被的全长重组CD 23与转染CD 11b-CD 18或CD 11 c-CD 18的COS细胞结合，而与转染CD 11 a-CD 18的COS细胞不结合。抗CD 11b或抗CD 11 c及抗CD 23单克隆抗体均能特异性抑制这种相互作用。通过用重组CD 23或抗CD 11b和抗CD 11 c单克隆抗体触发单核细胞上的CD 11b和CD 11 e，导致亚硝酸盐氧化产物和促炎细胞因子（IL-1 β、IL-6和TNF α）显著增加，证明了这种配体配对的功能意义。这些CD 23介导的活性被针对CD 11b、CD 11 c和CD 23的MAb的Fab片段降低。这些结果表明，CD 11b和CD 11 c是CD 23的受体，这种新的配体配对调节单核细胞的重要活动。
CD23 is expressed on a variety of haemopoietic cells and displays pleiotropic activities in vitro. We report that in addition to CD21 and IgE, CD23 interacts specifically with the CD11b and CD11c, the alpha chains of the beta 2 integrin adhesion molecule complexes CD11b-CD18 and CD11c-CD18, on monocytes. Full-length recombinant CD23 incorporated into fluorescent liposomes was shown to bind to COS cells transfected with cDNA encoding either CD11b-CD18 or CD11c-CD18 but not with CD11a-CD18, The interactio n was specifically inhibited by anti-CD11b or anti-CD11c, respectively, and by anti-CD23 MAbs. The functional significance of this ligand pairing was demonstrated by triggering CD11b and CD11e on monocytes with either recombinant CD23 or anti-CD11b and anti-CD11c MAbs to cause a marked increase in nitrite-oxidative products and proinflammatory cytokines (IL-1 beta, IL-6, and TNF alpha). These CD23-mediated activities were decreased by Fab fragments of MAbs to CD11b, CD11c, and CD23. These results demonstrate that CD11b and CD11c are receptors for CD23 and that this novel ligand pairing regulates important activities of monocytes.