Immune reactions of lymphocytes and macrophages against PEG-grafted pancreatic islets
Immune reactions of lymphocytes and macrophages against PEG-grafted pancreatic islets
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DOI：
10.1016/j.biomaterials.2003.10.062
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Byun, Y
中科院分区：
文献类型：
--
作者：
Jang, JY;Lee, DY;Byun, Y
移植物排斥是胰岛移植的主要限制因素，与针对移植物的T细胞和巨噬细胞的募集和激活密切相关。为了降低胰岛的免疫原性，我们将生物相容性聚乙二醇（PEG）移植到胰岛的胶原蛋白胶囊上，而不改变胰岛的形态和功能。在本研究中，我们评估了胰岛胶原蛋白被膜上移植的PEG分子是否可以阻止免疫细胞的活化，并研究了与体外免疫反应主要相关的因素。在与淋巴细胞共培养过程中，PEG接枝胰岛的形态和活力未受到破坏，与PEG接枝胰岛共培养的淋巴细胞分泌的IL-2和TNF-α量显着低于游离胰岛。然而，当两种胰岛与巨噬细胞一起培养时，其形态、活力以及细胞因子和一氧化氮的分泌量没有显着差异。总之，移植的 PEG 可以抑制淋巴细胞的活化，这对于启动移植物排斥过程至关重要。然而，嫁接的PEG分子并不能完全阻止细胞毒性分子渗入胰岛。 (C) 2003 Elsevier Ltd. 保留所有权利。
Graft rejection is the major limiting factor in islet transplantation and is closely related with the recruitment and activation of T cells and macrophages against the graft. To reduce the immunogenicity of islets, we have grafted biocompatible polyethylene glycol (PEG) onto the collagen capsule of islets without changing the morphology and function of islets. In this study, we evaluated whether the grafted PEG molecules on the collagen capsule of islet could prevent the activation of immune cells, and investigated factors that are mainly related to the immune reaction in vitro. During the co-culture with lymphocytes, the morphology and viability of PEG-grafted islets were not damaged, and the amounts of IL-2 and TNF-alpha secreted from lymphocytes co-cultured with PEG-grafted islets were significantly lower than that of free islets. However, when both kinds of islets were cultured with macrophages, there were no significant differences in morphology, viability and the secreted amounts of cytokines and nitric oxide. In conclusion, the grafted PEG could inhibit activation of lymphocytes, which are essential in initiating the graft rejection process. However, the grafted PEG molecules could not completely prevent the infiltration of cytotoxic molecules into the islets. (C) 2003 Elsevier Ltd. All rights reserved.