Identification of novel chicken microRNAs and analysis of their genomic organization.
Identification of novel chicken microRNAs and analysis of their genomic organization.
复制标题
DOI：
10.1016/j.gene.2008.04.004
复制
发表时间：
2008-07
期刊：
影响因子：
3.5
通讯作者：
Peng Shao;Hui Zhou;Zhen-Dong Xiao;Jie-hua He;Mian-bo Huang;Yue‐Qin Chen;L. Qu
中科院分区：
文献类型：
--
作者：
Peng Shao;Hui Zhou;Zhen-Dong Xiao;Jie-hua He;Mian-bo Huang;Yue‐Qin Chen;L. Qu
microRNAs（miRNAs）是一类非编码小分子RNA家族，在从细胞分化到生物体发育的多种生物学过程中发挥重要的调控作用。与其他脊椎动物相比，鸡的整套miRNAs及其在发育过程中的表达模式仍然知之甚少。在这项研究中，我们确定了29个新的miRNA和140个潜在的miRNA位点在鸡基因组中结合实验和计算分析。首先通过北方印迹分析了49个miRNAs的详细表达模式，并表明了miRNAs表达与其在胚胎发生和器官发生中的功能的协同性。系统地评估了鸡基因组中的27个miRNA簇，观察到紧密连锁的miRNA的不同表达模式。我们的研究结果显著扩展了鸡中已知的miRNA的集合，并为理解脊椎动物中miRNA基因的结构和功能进化提供了基础。
MicroRNAs (miRNAs) represent a family of small noncoding RNAs with important regulatory roles in diverse biological processes ranging from cell differentiation to organism development. In chickens, the full set of miRNAs and the expression patterns of miRNAs during development are still poorly understood when compared to the other vertebrates. In this study, we identified 29 novel miRNAs and 140 potential miRNA loci in the chicken genome by combining the experimental and computational analyses. Detailed expression patterns of 49 miRNAs were first characterized by Northern blotting and indicated the cooperativity of the miRNA expression with their function in embryogenesis and organogenesis. Twenty-seven miRNA clusters were systematically evaluated in the chicken genome and diverse expression patterns for closely linked miRNAs were observed. Our results significantly expand the set of known miRNAs in the chicken and provide the basis for understanding the structural and functional evolution of miRNA genes in vertebrates.