Diversity of endophytic diazotrophs isolated from Oryza alta
Diversity of endophytic diazotrophs isolated from Oryza alta
复制标题
稻内生固氮菌的多样性
DOI：
10.17520/biods.2019366
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发表时间：
2020
影响因子：
--
通讯作者：
Zhiyuan Tan
中科院分区：
文献类型：
--
作者：
Liping Liu;Ruifeng Song;Fu Zhang;Xiu;G. Peng;Zhiyuan Tan
：从野生稻 Oryza alta 中获得 43 株内生固氮细菌，并在无氮培养基上生长。通过乙炔还原法和 nifH 基因片段的 PCR 扩增测定了它们潜在的固氮能力。根据 IS-PCR 的 DNA 模式和 SDS-PAGE 的蛋白质模式，将获得的分离株分为六组（I、II、III、IV、V、VI）。 16S rRNA基因序列分析鉴定出代表性菌株，提示I组属于Raoultella terrigena，II组属于Klebsiella quasipneumoniae subsp。准肺炎杆菌，第III组为越南伯克霍尔德杆菌，第IV组为肠杆菌属，第V组为门多萨假单胞菌，第VI组为固氮植物杆菌。 Biolog 平板测试显示，不同组的菌株在碳源利用方面存在显着差异，通常在 IS-PCR 和 SDS-PAGE 模式中一致聚类。野生稻内生固氮细菌的多样性表明内生细菌从不同环境中获取碳和氮的适应性很强。
: Forty-three strains of endophytic nitrogen-fixing bacteria were obtained from wild rice Oryza alta , and grown on nitrogen-free medium. Their potential nitrogen-fixing ability was determined with the acetylene reduction method and a PCR amplification of the nifH gene fragment. The obtained isolates were grouped into six groups (I, II, III, IV, V, VI) based on their DNA patterns of IS-PCR and protein patterns of SDS-PAGE. The 16S rRNA gene sequence analysis identified representative strains, suggesting that group I belonged to Raoultella terrigena , group II to Klebsiella quasipneumoniae subsp . quasipneumoniae , group III to Burkholderia vietnamiensis , group IV to genus Enterobacter , group V to Pseudomonas mendocina , and group VI to Phytobacter diazotrophicus . A Biolog plate test showed strains from differing groups had significant differences in carbon source utilization, often clustering consistently within IS-PCR and SDS-PAGE patterns. The diversity of endophytic nitrogen-fixing bacteria in wild rice indicates that endophytic bacteria is strongly adaptable in obtaining both carbon and nitrogen from varying environments.