Molecular cloning and sequence determination of a cDNA coding for the alpha-subunit of a Go-type protein of Xenopus laevis oocytes.
Molecular cloning and sequence determination of a cDNA coding for the alpha-subunit of a Go-type protein of Xenopus laevis oocytes.
复制标题
非洲爪蟾卵母细胞 Go 型蛋白 α 亚基编码 cDNA 的分子克隆和序列测定。
DOI：
10.1016/0014-5793(89)81190-1
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发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
Allende,JE
中科院分区：
文献类型：
--
作者：
Olate,J;Jorquera,H;Purcell,P;Codina,J;Birnbaumer,L;Allende,JE
非洲爪蟾卵母细胞是研究信号转导和参与这一过程的g蛋白的理想细胞。斧子。λgt10的laeviscDNA文库用三种寡核苷酸探针的混合物进行了筛选，这些探针设计用于检测各种哺乳动物g蛋白α-亚基的序列。其中一个克隆通过三级筛选得到纯化，并对插入的DNA进行了测序。该克隆包含一个354个氨基酸的蛋白质的总序列，与大鼠Go α-亚基的序列相同89%。与哺乳动物蛋白的差异集中在290-315个氨基酸上，这些氨基酸被认为是与受体和效应分子相互作用的区域。与其他哺乳动物g蛋白α-亚基的同源性较低，分别为65-70%和42%。在此基础上，该克隆可归类为Go-like。
Xenopus laevisoocytes are cells ideally suited to the study of signal transduction and of the G-proteins that are involved in this process. AX. laeviscDNA library in λgt10 has been screened with a mixture of three oligonucleotide probes designed to detect sequences found in various mammalian α-subunits of G-proteins. One of these clones has been purified through tertiary screening and the DNA insert has been sequenced. This clone was found to include the total sequence coding for a 354 amino acid protein that is 89% identical to the sequence of α-subunit of rat Go. The differences with the mammalian protein were clustered in amino acids 290–315, which have been postulated to define the region interacting with the receptor and effector molecule. The homology with the α-subunits of other mammalian G-proteins is lower (65–70% to Giand 42% to Gs). On this basis, this clone can be classified as Go-like.