Cytotoxicity of carbon nanomaterials: Single-wall nanotube, multi-wall nanotube, and fullerene
Cytotoxicity of carbon nanomaterials: Single-wall nanotube, multi-wall nanotube, and fullerene
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DOI：
10.1021/es048729l
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发表时间：
2005-03-01
影响因子：
11.4
通讯作者：
Guo, XB
中科院分区：
文献类型：
--
作者：
Jia, G;Wang, HF;Guo, XB
对单壁纳米管（SWNTs）、多壁纳米管（直径范围为10到20纳米，MWNT10）以及富勒烯（C - 60）的细胞毒性测试方案进行了测试。在体外暴露6小时后，在肺泡巨噬细胞（AM）中观察到单壁纳米管具有显著的细胞毒性。当单壁纳米管的剂量增加11.30微克/平方厘米时，细胞毒性增加高达约35%。在剂量高达226.00微克/平方厘米时，未观察到C60有明显毒性。从质量基础上看，细胞毒性明显遵循以下顺序：单壁纳米管>多壁纳米管10>石英>C - 60。单壁纳米管在低剂量0.38微克/平方厘米时就显著损害肺泡巨噬细胞的吞噬作用，而多壁纳米管10和C60仅在高剂量3.06微克/平方厘米时才引发损伤。暴露于3.06微克/平方厘米的单壁纳米管或多壁纳米管10的巨噬细胞呈现出坏死和变性的特征。可能存在凋亡细胞死亡的迹象。不同几何结构的碳纳米材料在体外表现出截然不同的细胞毒性和生物活性，尽管它们在体内的相对毒性可能无法准确反映。
A cytotoxicity test protocol for single-wall nanotubes (SWNTs), multi-wall nanotubes (with diameters ranging from 10 to 20 nm, MWNT10), and fullerene (C-60) was tested. Profound cytotoxicity of SWNTs was observed in alveolar macrophage (AM) after a 6-h exposure in vitro. The cytotoxicity increases by as high as similar to35% when the dosage of SWNTs was increased by 11.30 mug/cm(2). No significant toxicity was observed for C60 up to a dose of 226.00 mug/CM2. The cytotoxicity apparently follows a sequence order on a mass basis: SWNTs > MWNT10 > quartz > C-60. SWNTs significantly impaired phagocytosis of AM at the low dose of 0.38 mug/cm(2), whereas MWNT10 and C60 induced injury only at the high dose of 3.06 mug/cm(2). The macrophages exposed to SWNTs or MWNT10 of 3.06 mug/cm(2) showed characteristic features of necrosis and degeneration. A sign of apoptotic cell death likely existed. Carbon nanomaterials with different geometric structures exhibit quite different cytotoxicity and bioactivity in vitro, although they may not be accurately reflected in the comparative toxicity in vivo.