Metalloporphyrin-based porous polymers prepared via click chemistry for size-selective adsorption of protein
Metalloporphyrin-based porous polymers prepared via click chemistry for size-selective adsorption of protein
复制标题
通过点击化学制备金属卟啉基多孔聚合物，用于蛋白质的尺寸选择性吸附
DOI：
10.1080/09205063.2018.1456025
复制
发表时间：
2018-01-01
影响因子：
3.6
通讯作者：
Ye, Peng
中科院分区：
文献类型：
--
作者：
Zhu, Dailian;Qin, Cunqi;Ye, Peng
摘要通过控制点击化学反应条件，制备了不同孔径的锌卟啉基多孔聚合物（PPs-Zn），并研究了PPs-Zn对蛋白质的吸附和固定化酶的催化活性。通过FTIR、NMR和氮气吸附实验对孔径为18 nm和90 nm的PPS-Zn-1和PPS-Zn-2进行了表征。PPs-Zn-1对溶菌酶、脂肪酶和牛血清白蛋白（BSA）等小分子蛋白质的吸附量大于PPs-Zn-2。对于大分子蛋白质，包括肌球蛋白和人纤维蛋白原（HFg），PPs-Zn-1的吸附量小于PPs-Zn-2。结果表明，PPs-Zn对蛋白质的吸附具有尺寸选择性。蛋白质的大小和孔径大小对PPs-Zn中蛋白质的吸附量有显著影响。PPs-Zn固定化脂肪酶和溶菌酶具有良好的重复使用稳定性。
Abstract Zinc porphyrin-based porous polymers (PPs-Zn) with different pore sizes were prepared by controlling the reaction condition of click chemistry, and the protein adsorption in PPs-Zn and the catalytic activity of immobilized enzyme were investigated. PPs-Zn-1 with 18 nm and PPS-Zn-2 with 90 nm of pore size were characterized by FTIR, NMR and nitrogen absorption experiments. The amount of adsorbed protein in PPs-Zn-1 was more than that in PPs-Zn-2 for small size proteins, such as lysozyme, lipase and bovine serum albumin (BSA). And for large size proteins including myosin and human fibrinogen (HFg), the amount of adsorbed protein in PPs-Zn-1 was less than that in PPs-Zn-2. The result indicates that the protein adsorption is size-selective in PPs-Zn. Both the protein size and the pore size have a significant effect on the amount of adsorbed protein in the PPs-Zn. Lipase and lysozyme immobilized in PPs-Zn exhibited excellent reuse stability.