NUCLEAR PROTEINS .3. FIBRILLAR NATURE OF NUCLEAR MATRIX
NUCLEAR PROTEINS .3. FIBRILLAR NATURE OF NUCLEAR MATRIX
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DOI：
10.1016/0014-4827(76)90271-8
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发表时间：
1976-01-01
影响因子：
3.7
通讯作者：
OKADA, TA
中科院分区：
文献类型：
--
作者：
COMINGS, DE;OKADA, TA
用高盐、DNase和Triton X-100提取小鼠肝核染色质，检测核基质。残核基质由核孔层复合物、纤维状核仁和核内基质组成。整体电镜显示，部分核基质由20-30 . ang组成。称为基质蛋白的蛋白质纤维。纤维可以结合形成更大的100-300 . ang。纤维。当使用小鼠睾丸细胞时，完整的突触复合物和性囊泡与基质密切相关，可能由基质素组成。SDS凝胶电泳显示了三种主要的多肽65,000,67,000和68,000 D.一些观察表明DNA在整个细胞核的许多位置附着在基质上。基质可能在染色质进入减数分裂和有丝分裂染色体的染色质排列中起作用。
The nuclear matrix of mouse liver nuclei was examined after extraction of the chromatin with high salt, DNase and Triton X-100. The residual nuclear matrix is composed of a nuclear pore-lamina complex, fibrillar nucleoli and intranuclear matrix. Whole mount EM shows that a portion of the nuclear matrix is composed of 20-30 .ANG. protein fibers called matrixin. The fibers may associate to form larger 100-300 .ANG. fibers. When mouse testicular cells were used, intact synaptonemal complexes and the sex vesicle were intimately associated with the matrix and may be composed of matrixin. SDS gel electrophoresis of the matrix shows 3 major polypeptides of 65,000, 67,000 and 68,000 D. Several observations suggest DNA is attached to the matrix at many sites throughout the nucleus. The matrix may play a role in the arrangement of chromatin into the chromomeres of meiotic and mitotic chromosomes.