DIRECT MAGNETIC SEPARATION OF RED-CELLS FROM WHOLE-BLOOD
DIRECT MAGNETIC SEPARATION OF RED-CELLS FROM WHOLE-BLOOD
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DOI：
10.1038/255706a0
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发表时间：
1975-01-01
期刊：
影响因子：
64.8
通讯作者：
ROATH, S
中科院分区：
文献类型：
--
作者：
MELVILLE, D;PAUL, F;ROATH, S
血红蛋白的磁性是公认的。血红蛋白的修复基具有铁原子被离子结合的原卟啉结构(铁血红素)。因此，在脱氧血红蛋白中，每个铁原子有四个未配对的电子，导致每个血红蛋白基团的顺磁矩为5.35个玻尔磁子。另一方面，氧合血红蛋白是抗磁性的。其他含铁的血蛋白，如高铁血红蛋白，已经通过磁化率测量2显示出每个血球蛋白都有磁矩，这与根据每个铁原子的未配对电子数预测的磁矩相当接近。因此，当含有脱氧血红蛋白的红细胞被置于具有磁场梯度dB0/dz的磁场B0(特斯拉)中时，可以预期在Z方向上经历由Fm=(χV/μ0)B0(dB0/dz)(1)给出的磁力Fm(牛顿)，其中χ是红细胞的(SI)敏感性，V(M3)其体积和μ0是自由空间的磁导率。
THE magnetic properties of haemoglobin are well established1. The prosthetic group of haemoglobin has a protoporphyrin structure (ferrohaem) in which the iron atom is ionically bound. Consequently there are in deoxyhaemoglobin four unpaired electrons per iron atom leading to a paramagnetic moment of 5.35 Bohr magnetons per haem group. Oxyhaemoglobin on the other hand is diamagnetic. Other iron-containing haemoproteins such as ferrihaemoglobin have been shown by magnetic susceptibility measurements2to possess magnetic moments per haem group which correspond reasonably closely to those predicted from the number of unpaired electrons per iron atom. Consequently red blood cells containing deoxyhaemoglobin when placed in a magnetic fieldB0(Tesla) with field gradient dB0/dzmay be expected to experience a magnetic forceFM(Newtons) in thezdirection given byFM= (χV/μ0)B0(dB0/dZ) (1) where χ is the (SI) susceptibility of the red blood cell,V(m3) its volume and μ0the permeability of free space.