Determination of vessel cross-sectional area by thresholding in Radon space
Determination of vessel cross-sectional area by thresholding in Radon space
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DOI：
10.1038/jcbfm.2014.67
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发表时间：
2014-07-01
影响因子：
6.3
通讯作者：
Drew, Patrick J.
中科院分区：
文献类型：
--
作者：
Gao, Yu-Rong;Drew, Patrick J.
血管的横截面积决定了它的阻力，因此是局部血流的调节器。然而，皮层穿透血管的横截面可以是非圆形的，扩张和收缩可以改变血管的形状。我们表明，当使用单一直径测量时，观察到的容器形状变化会在通量计算中引入较大的误差。由于这些形状变化，典型的直径测量方法，如依赖于单个直径轴的半最大全宽度（FWHM）将产生错误的结果，特别是在计算通量时。在这里，我们提出了一种自动方法-氡空间阈值(TiRS)-用于确定凸物体的横截面积，例如用双光子激光扫描显微镜（2PLSM）观察到的穿透容器。将阈值化后的图像转换回图像空间，并对相邻像素进行分割。TiRS方法类似于在多个轴上进行FWHM，并且比FWHM和阈值方法对噪声和形状变化具有更强的鲁棒性。我们证明了TiRS方法在体内2PLSM测量血管直径的优越精度。
The cross-sectional area of a blood vessel determines its resistance, and thus is a regulator of local blood flow. However, the cross-sections of penetrating vessels in the cortex can be non-circular, and dilation and constriction can change the shape of the vessels. We show that observed vessel shape changes can introduce large errors in flux calculations when using a single diameter measurement. Because of these shape changes, typical diameter measurement approaches, such as the full-width at half-maximum (FWHM) that depend on a single diameter axis will generate erroneous results, especially when calculating flux. Here, we present an automated method-thresholding in Radon space (TiRS)-for determining the cross-sectional area of a convex object, such as a penetrating vessel observed with two-photon laser scanning microscopy (2PLSM). The thresholded image is transformed back to image space and contiguous pixels are segmented. The TiRS method is analogous to taking the FWHM across multiple axes and is more robust to noise and shape changes than FWHM and thresholding methods. We demonstrate the superior precision of the TiRS method with in vivo 2PLSM measurements of vessel diameter.