Calorie restriction-induced maternal longevity is transmitted to their daughters in a rotifer
Calorie restriction-induced maternal longevity is transmitted to their daughters in a rotifer
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DOI：
10.1111/j.1365-2435.2010.01773.x
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发表时间：
2011-02-01
影响因子：
5.2
通讯作者：
Watabe, Shugo
中科院分区：
文献类型：
--
作者：
Kaneko, Gen;Yoshinaga, Tatsuki;Watabe, Shugo
食物短缺导致的寿命延长，通常在实验室中被卡路里限制（CR）所模拟，是真核生物最常见的生活史改变之一。虽然后代的生活史往往会随着亲代环境的变化而发生变化，但cr诱导的长寿是否会遗传给下一代，目前还不清楚本研究研究了CR对孤雌轮虫（Brachionus plicatilis）寿命、氧化应激抗性以及过氧化氢酶和锰超氧化物歧化酶（Mn SOD）两种抗氧化酶表达水平的影响CR处理下的轮虫寿命比随意喂食的轮虫长50%，这与抗氧化应激能力的增强以及过氧化氢酶和Mn sod mRNA水平的升高有关。4无论食物丰富程度和CR条件如何，CR处理的母鼠的后代比随意喂食AL的母鼠的后代寿命长20%。此外，cr处理的母亲的后代在出生时具有更高的抗氧化应激能力，过氧化氢酶mRNA水平升高，但Mn SOD水平不高。与mRNA的表达模式一致，CR增加了卵和母鼠全身过氧化氢酶和Mn SOD的蛋白水平我们对这些无性繁殖轮虫的研究结果提供了第一个证据，证明CR导致的长寿和抗氧化应激能力可以遗传给后代。
P>1 Extension of life span by food shortage, often mimicked by calorie restriction (CR) in the laboratory, is one of the most common life-history alterations in eukaryotes. Although the life-history of offspring often changes in response to the parental environment, it has remained ambiguous whether or not CR-induced longevity is transmitted to the next generation.2 Here, we investigated the effects of CR on life span, oxidative stress resistance and the expression levels of two antioxidant enzymes, catalase and manganese superoxide dismutase (Mn SOD), in the parthenogenetic rotifer Brachionus plicatilis during two consecutive generations.3 Rotifers under CR lived 50% longer than those fed ad libitum (AL) in association with enhancement of oxidative stress resistance and increased mRNA levels of catalase and Mn SOD.4 The daughters from the CR-treated mothers lived 20% longer than those from the mothers fed AL regardless of food-rich and CR conditions for the daughter. Furthermore, the daughters from the CR-treated mothers were endowed at birth with a higher ability to resist oxidative stress and the increased mRNA levels for catalase, but not for Mn SOD. In agreement with the mRNA expression patterns, CR increased the protein levels of catalase and Mn SOD in eggs and the whole body of mothers, respectively.5 Our results for these asexually reproducing rotifers provide the first evidence that the longevity and oxidative stress resistance resulting from CR can be passed on to subsequent generations.