Pre-eclampsia is associated with the failure of melanoma cell adhesion molecule (MCAM/CD146) expression by intermediate trophoblast
Pre-eclampsia is associated with the failure of melanoma cell adhesion molecule (MCAM/CD146) expression by intermediate trophoblast
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先兆子痫与中间滋养层黑色素瘤细胞粘附分子（MCAM/CD146）表达失败有关
DOI：
10.1038/labinvest.3700033
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发表时间：
2004-02-01
影响因子：
5
通讯作者：
Shen, Y
中科院分区：
文献类型：
--
作者：
Liu, Q;Yan, XY;Shen, Y
本研究的目的是确定黑色素瘤细胞粘附分子（MCAM/CD 146）的表达，像大多数其他内皮细胞粘附分子，是减少在妊娠期疾病先兆子痫，以及是否与滋养层细胞的侵袭性。我们使用免疫组化方法，并分析了42个胎盘从控制和先兆子痫患者使用抗CD 146单克隆抗体。我们的数据显示，在正常胎盘，CD 146染色是专门检测到中间滋养层细胞，最具侵袭性和迁移性，但没有检测到非侵袭性细胞滋养层细胞或合体滋养层。然而，在先兆子痫中，CD 146不再存在于中间滋养层中，尽管在血管中可检测到。同时，我们检测了另一种内皮细胞粘附分子CD 31/PECAM-1，发现其对正常和先兆子痫胎盘中的各种滋养细胞均呈阴性染色。正常胎盘和先兆子痫胎盘中CD 146的不同染色模式提供了第一个证据，即在先兆子痫中，中间滋养细胞不能表达CD 146，暗示CD 146在滋养细胞侵袭中起重要作用。
The purpose of this study was to determine whether the expression of melanoma cell adhesion molecule (MCAM/CD146), like most other endothelial adhesion molecules, is reduced in the pregnancy disorder pre-eclampsia, and whether it is associated with the invasiveness of trophoblast. We used immunohistochemical approaches and analyzed 42 placentas from control and pre-eclamptic patients using an anti-CD146 monoclonal antibody. Our data show that in normal placentas, CD146 staining was specifically detected in intermediate trophoblasts that are most invasive and migratory, but not detected in noninvasive cytotrophoblasts or syncytiotrophoblasts. However in pre-eclampsia, CD146 was no longer present in the intermediate trophoblasts, although it was detectable in the blood vessels. At the same time, we tested CD31/PECAM-1, another endothelial adhesion molecule, and found its negative staining for all kinds of trophoblasts in both normal and pre-eclamptic placentas. The different staining patterns of CD146 in the normal and pre-eclamptic placentas provide the first evidence that in pre-eclampsia, intermediate trophoblasts fail to express CD146, implicating that CD146 plays an important role in trophoblast invasion.