QTL Analysis of Morphological, Developmental, and Winter Hardiness-Associated Traits in Perennial Ryegrass
QTL Analysis of Morphological, Developmental, and Winter Hardiness-Associated Traits in Perennial Ryegrass
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DOI：
10.2135/cropsci2004.9250
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发表时间：
2004-05
期刊：
影响因子：
2.3
通讯作者：
Toshihiko Yamada;Elizabeth S. Jones;N. Cogan;A. Vecchies;T. Nomura;H. Hisano;Y. Shimamoto;Kevin F. Smith;M. D. Hayward;J. Forster
中科院分区：
文献类型：
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作者：
Toshihiko Yamada;Elizabeth S. Jones;N. Cogan;A. Vecchies;T. Nomura;H. Hisano;Y. Shimamoto;Kevin F. Smith;M. D. Hayward;J. Forster
对多年生黑麦草（Lolium perenne L.）通过基于参考分子标记的遗传图谱进行鉴定。复制表型数据获得了一些实地评估的形态和发育性状，以及冬季生存和电导率的抗寒性相关的字符。标记性状关联分析进行了一些方法，并观察到高度的一致性之间的各自的结果。检测了株高、分蘖大小、叶长、叶宽、收获时鲜重、株型、单穗小穗数、穗长等形态性状以及抽穗期和抽穗后程度等发育性状的QTL。许多性状显著相关，并确定了一致的QTL位置。未发现影响田间越冬率的显著QTL。然而，电导率对应的耐霜冻QTL位于接近抽穗期QTL的区域，可能显示保守的同线性与染色体区域相关的抗寒性和开花时间的变化在谷物。多个表型性状的QTL分析为多年生黑麦草重要农艺性状的标记辅助选择（MAS）提供了基础，为多年生黑麦草产量、品质和适应性的遗传改良提供了可能。
Quantitative trait loci (QTLs) for a number of agronomically important traits of perennial ryegrass (Lolium perenne L.) were identified by means of a reference molecular marker-based genetic map. Replicated phenotypic data was obtained for a number of field-assessed morphological and developmental traits as well as the winter hardiness-associated characters of winter survival and electrical conductivity. Marker-trait association analysis was performed by a number of methods, and a high degree of congruence was observed between the respective results. QTLs were detected for morphological traits such as plant height, tiller size, leaf length, leaf width, fresh weight at harvest, plant type, spikelet number per spike and spike length, as well as the developmental traits of heading date and degree of aftermath heading. A number of traits were significantly correlated, and coincident QTL locations were identified. No significant QTLs for winter survival in the field were identified. However, a QTL for electrical conductivity corresponding to frost tolerance was located close to a heading date QTL in a region that may show conserved synteny with chromosomal regions associated with both winter hardiness and flowering time variation in cereals. The QTL analysis of multiple phenotypic traits provides the basis for marker assisted selection (MAS) of important agronomic characters, allowing genetic improvement of yield, quality and adaptation in perennial ryegrass breeding.