Design of larval chemical attractants based on odorant response spectra of odorant receptors in the cotton bollworm
Design of larval chemical attractants based on odorant response spectra of odorant receptors in the cotton bollworm
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基于棉铃虫气味受体气味反应谱的幼虫化学引诱剂设计
DOI：
10.1016/j.ibmb.2017.03.007
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发表时间：
2017-05-01
影响因子：
3.8
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
--
作者：
Di, Chang;Ning, Chao;Wang, Chen-Zhu
鳞翅目毛虫依靠嗅觉和味觉辨别食物来源。与幼虫的味觉相比，对幼虫嗅觉的研究很少。为了揭示棉铃虫幼虫嗅觉的分子基础，我们利用转录组测序技术鉴定了棉铃虫触角和上颚的17个气味受体（Or）基因，并对7个Or对生态相关气味的反应谱进行了功能鉴定。在趋化性试验中评估了幼虫对活性气味剂的先天行为反应。基于Ors调谐光谱和毛虫趋化性，配制了几种气味共混物。一种四组分混合物对棉铃虫幼虫有较强的吸引力，其中顺式茉莉素和1-戊醇为主要成分。定位分析表明，检测这些成分的两个or （Or41和Or52）在相同的感觉神经元中表达。这是第一个证明多食性毛虫对气味有组合反应的证据。设计针对特定嗅觉通路的引诱剂可以促进害虫防治新毒饵的开发。(C) 2017 Elsevier Ltd.版权所有。
Lepidopteran caterpillars rely on olfaction and gustation to discriminate among food sources. Compared to the larval gustation, the larval olfaction has been poorly investigated. To uncover the molecular basis of olfaction in Helicoverpa armigera larvae, we identified 17 odorant receptor (Or) genes in larval antennae and maxillae using transcriptome sequencing, and functionally identified the response spectra of seven Ors to ecologically relevant odorants. Innate behavioural responses of larvae to active odorants were evaluated in chemotaxis assays. Several odorant blends were formulated based on the Ors tuning spectra and caterpillar chemotaxis. A four-component blend strongly attracted H. armigera larvae, and cis-jasmone and 1-pentanol were identified as essential components. Localization analyses showed that the two Ors detecting these components (Or41 and Or52) were expressed in the same sensory neurons. This is the first evidence that Ors in a polyphagous caterpillar respond to odorants in a combinatorial manner. The design of attractants to target specific olfactory pathways may promote the development of new baits for pest management. (C) 2017 Elsevier Ltd. All rights reserved.