Assessing behavioral toxicity with Caenorhabditis elegans
Assessing behavioral toxicity with Caenorhabditis elegans
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DOI：
10.1897/03-264
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发表时间：
2004-05-01
影响因子：
4.1
通讯作者：
Williams, PL
中科院分区：
文献类型：
--
作者：
Anderson, GL;Cole, RD;Williams, PL
行为，即使在简单的后生动物中，也依赖于亚细胞、细胞和有机体水平的综合过程，因此很容易受到广泛化学物质的干扰。小型自由生活线虫秀丽线虫的运动行为(运动)已被证明在评估毒性方面是有用的。最近报道的观察表明，重金属暴露4h后，行为改变(运动减少)发生，而在短时间暴露下，铅(一种已知的神经毒性金属)的浓度-反应关系不同于铜。在这项研究中，评估了来自三个化学类别：有机农药、有机溶剂和重金属的九种化合物在暴露4小时后的迁移情况。除甲苯咪唑外，所有受试化合物都观察到了浓度依赖性的运动减少。其测试浓度受溶解度的限制。在每个化学类别中，运动对神经毒性化合物比对不被认为是神经毒性的物质更敏感，行为有效浓度证明了这一点，将平均蠕虫运动减少到对照运动值的50%(例如，左旋咪唑和毒死蜱和甲苯咪唑)。乙醇和丙酮及二甲基亚砜。和Pb和Al<；Cu)。这些观察结果与急性行为测试在评估一般化学毒性中的使用以及4小时测试在检测神经毒物方面的增强价值有关。
Behavior, even in simple metazoans, depends upon integrated processes at the subcellular, cellular, and organismal level, and thus is susceptible to disruption by a broad spectrum of chemicals. Locomotor behavior (movement) of the small free-living nematode Caenorhabditis elegans has proven to be useful in assessing toxicity. Recently reported observations suggest that behavioral change (reduced movement) occurs after 4 h of exposure to heavy metals, and that with abbreviated exposure, the concentration-response relationship for Pb (a known neurotoxic metal) differs from that for Cu. In this Study, movement was evaluated after 4-h exposures for nine compounds from three chemical classes: organic pesticides, organic solvents, and heavy metals. Concentration-dependent reduction of movement was observed for all test compounds with the exception of mebendazole. for which test concentrations were limited by solubility. Within each chemical class, movement was more sensitive to the neurotoxic compounds than to substances not believed to be neurotoxic, Lis evidenced by behavioral effective concentration to reduce average worm movement to 50% of the control movement values (e.g., levamisole and chlorpyrifos < mebendazole. ethanol and acetone < dimethylsulfoxide. and Pb and Al < Cu). These observations are discussed as they relate to the Use Of acute behavioral tests in assessing general chemical toxicity, and the enhanced Value of 4-h testing for the detection of neural toxicants.