Structural modulation of gut microbiota during alleviation of antibiotic-associated diarrhea with herbal formula
Structural modulation of gut microbiota during alleviation of antibiotic-associated diarrhea with herbal formula
复制标题
用草药配方缓解抗生素相关腹泻期间肠道微生物群的结构调节
DOI：
10.1016/j.ijbiomac.2017.02.060
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发表时间：
2017-12-01
影响因子：
8.2
通讯作者：
Guo, Shining
中科院分区：
文献类型：
--
作者：
Lv, Weijie;Liu, Cui;Guo, Shining
肠道微生物组被假设在胃肠道疾病中发挥关键作用，包括抗生素相关性腹泻（AAD）。为了确定传统中药复方参苓白术散（SLBZS）是否在AAD治疗期间调节肠道微生物组的组成，通过连续7天灌胃林可霉素，然后施用SLBZS一周，在大鼠中制备AAD腹泻模型。SLBZS治疗后各时间点的腹泻率均显著低于模型组，或至少在数值上低于模型组。SLBZS治疗后发生了肠道微生物组的整体结构调节，对MD诱导的结构变化具有恢复作用。在属水平上，Sutterella的相对丰度与SLBZS处理呈负相关，与未处理呈正相关，表明Sutterella可能是改善MD的关键类群。响应SLBZS的肠道微生物组的关键细菌类型表明有益细菌的富集，特别是拟杆菌属。因此，这些数据表明，肠道微生物组的结构变化是由中草药配方SLBZS诱导的。总之，肠道微生物组的变化与SLBZS的肠道控制作用有关。(C)2017爱思唯尔B.V.保留所有权利。
The gut microbiome is hypothesized to play a critical role in gastrointestinal diseases, including antibiotic associated diarrhea (AAD). To determine whether the traditional Chinese herbal formula of Shen Ling Bai Zhu San (SLBZS) modulates the composition of the gut microbiome during AAD treatment, an AAD diarrhea model was prepared in rats by gastric gavage with lincomycin for 7 successive days, followed by administration of SLBZS for one week. At all time points after the SLBZS treatment, the diarrhea rates were significantly or at least numerically lower than that of the untreated model group. Overall structural modulation of the gut microbiome occurred after SLBZS treatment, with reverting effects on the MD-induced structural variations. At the genus level, the relative abundance of Sutterella was negatively correlated with SLBZS treatment and positively correlated with a lack of treatment, suggesting that Sutterella might be a pivotal phylotype associated with the improvement of MD. The key phylotypes of the gut micro-biome that responded to SLBZS indicated enrichment of beneficial bacteria, and particularly Bacteroides spp. These data therefore demonstrated that structural changes of the gut microbiome are induced by the Chinese herbal formula SLBZS. In conclusion, changes in the gut microbiome are associated with the diarrhea-controlling effect of SLBZS. (C) 2017 Elsevier B.V. All rights reserved.