Aspergillus oryzae nrtA affects kojic acid production
Aspergillus oryzae nrtA affects kojic acid production
复制标题
DOI：
10.1080/09168451.2016.1176517
复制
发表时间：
2016-09-01
影响因子：
1.6
通讯作者：
Sano, Motoaki
中科院分区：
文献类型：
--
作者：
Sano, Motoaki
我们通过基因破坏分析了米曲霉硝酸盐转运蛋白编码基因 (nrtA) 的作用。 Southern杂交分析表明同源重组发生在常驻nrtA基因座处。实时PCR表明nrtA基因可被NaNO3强烈诱导。当硝酸盐作为唯一氮源时，nrtA 破坏体没有表现出正常生长。这些结果表明 NrtA 对于米曲霉的硝酸盐吸收至关重要。添加少量硝酸钠可抑制曲酸（KA）的产生。 nrtA 破坏的菌株缺乏硝酸盐的吸收。因此，该菌株中 KA 的产生并未受到硝酸盐存在的显着影响。
We analyzed the role of the nitrate transporter-encoding gene (nrtA) of Aspergillus oryzae by gene disruption. Southern hybridization analysis indicated that homologous recombination occurred at the resident nrtA locus. Real-time PCR showed that the nrtA gene was strongly inducible by NaNO3. The nrtA disruptant did not exhibit normal growth when nitrate was available as the sole nitrogen source. These results indicate that NrtA is essential for nitrate uptake in A. oryzae. Kojic acid (KA) production was inhibited by the addition of a small amount of sodium nitrate. The nrtA-disrupted strain was deficient in the uptake of nitrate. As a result, KA production in this strain was not considerably affected by the presence of nitrate.