STIMULATION BY EGF OF THE GROWTH OF EGF RECEPTOR-HYPERPRODUCING TUMOR-CELLS IN ATHYMIC MICE
STIMULATION BY EGF OF THE GROWTH OF EGF RECEPTOR-HYPERPRODUCING TUMOR-CELLS IN ATHYMIC MICE
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DOI：
10.1002/ijc.2910400523
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发表时间：
1987-11-15
影响因子：
6.4
通讯作者：
SHIMIZU, N
中科院分区：
文献类型：
--
作者：
OZAWA, S;UEDA, M;SHIMIZU, N
皮下接种源自鳞状细胞癌的EGF受体高产细胞。将其植入无胸腺小鼠的双侧腹部区域，并在其背部植入含有 EGF 的微型渗透泵。 2周后，在EGF存在下由5种不同细胞系形成的肿瘤的重量比在不存在EGF的情况下形成的肿瘤重3至6倍。从这些肿瘤中恢复的细胞保持了其原始特征，例如扩增的 EGF 受体基因、大量的 EGF 受体以及对 EGF 介导的体外生长抑制的敏感性。这些结果表明EGF在促进体内鳞状细胞癌生长中发挥重要作用。
EGF receptor-hyperproducing cells of squamous carcinoma origin were inoculated s.c. into the bilatero-abdominal regions of athymic mice and a mini-osmotic pump containing EGF was implanted on the back. After 2 weeks the tumors formed from 5 different cell lines in the presence of EGF weighed 3 to 6 times more than those formed in the absence of EGF. The cells recovered from these tumors maintained their original characteristics such as the amplified EGF receptor gene, high numbers of EGF receptors and a sensitivity to EGF-mediated inhibition of growth in vitro. These results suggest that EGF plays an important role in promoting growth of squamous-cell carcinoma in vivo.