Hematopoietic stem cell-specific GFP-expressing transgenic mice generated by genetic excision of a pan-hematopoietic reporter gene.
Hematopoietic stem cell-specific GFP-expressing transgenic mice generated by genetic excision of a pan-hematopoietic reporter gene.
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DOI：
10.1016/j.exphem.2016.05.002
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发表时间：
2016-08
影响因子：
2.6
通讯作者：
Forsberg EC
中科院分区：
文献类型：
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作者：
Perez-Cunningham J;Boyer SW;Landon M;Forsberg EC
通过在造血系统内表达绿色荧光蛋白（GFP）来选择性标记特定细胞类型将在流式细胞术、显微术、谱系追踪和移植测定中识别、定位和追踪不同细胞群方面具有很大的实用性。在这份报告中，我们描述了一种新的转基因小鼠系的产生和表征，该系具有所有有核造血细胞以及血小板的特异性GFP标记。这种新的“Vav-GFP”小鼠系在胚胎发育和成年期间用GFP标记绝大多数造血细胞，在造血干细胞和祖细胞中表达特别高。除了胎儿内皮细胞的瞬时标记外，GFP表达对造血细胞具有高度选择性，并且在造血祖细胞移植后在供体来源的后代中持续存在。最后，我们还表明，loxP侧翼的报告允许特定的GFP标记不同的造血细胞亚群时，交叉到各种Cre报告线。值得注意的是，通过将Vav-GFP小鼠与Flk 2-Cre小鼠杂交，我们在HSC中获得了稳健且高度选择性的GFP表达。总之，这些数据描述了一种新的小鼠模型，能够指导GFP标记专门的造血细胞或专门的HSC。
Selective labeling of specific cell types by expression of green fluorescent protein (GFP) within the hematopoietic system would have great utility in identifying, localizing, and tracking different cell populations in flow cytometry, microscopy, lineage tracing, and transplantation assays. In this report, we describe the generation and characterization of a new transgenic mouse line with specific GFP labeling of all nucleated hematopoietic cells, as well as platelets. This new “Vav-GFP” mouse line labels the vast majority of hematopoietic cells with GFP during both embryonic development and adulthood, with particularly high expression in hematopoietic stem and progenitor cells. With the exception of transient labeling of fetal endothelial cells, GFP expression is highly selective for hematopoietic cells, and persists in donor-derived progeny after transplantation of hematopoietic progenitor cells. Finally, we also demonstrate that the loxP-flanked reporter allows for specific GFP labeling of different hematopoietic cell subsets when crossed to various Cre reporter lines. Notably, by crossing Vav-GFP mice to Flk2-Cre mice, we obtained robust and highly selective GFP expression in HSCs. Together, these data describe a new mouse model capable of directing GFP labeling exclusively of hematopoietic cells or exclusively of HSCs.
影响因子：
2.6
作者：
Duong, Khanh L.;Das, Satyabrata;Yu, Shuyang;Barr, Jennifer Y.;Jena, Snehalata;Kim, Eunmi;Zavazava, Nicolas;Colgan, John D.;Xue, Hai-Hui;Levasseur, Dana N.
通讯作者：
Levasseur, Dana N.