CYCLIC-AMP ANALOGS INCREASE EXCITABILITY AND ENHANCE EPILEPTIFORM ACTIVITY IN RAT NEOCORTEX INVITRO
CYCLIC-AMP ANALOGS INCREASE EXCITABILITY AND ENHANCE EPILEPTIFORM ACTIVITY IN RAT NEOCORTEX INVITRO
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DOI：
10.1016/0014-2999(93)90235-a
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发表时间：
1993-05-12
影响因子：
5
通讯作者：
OSHAUGHNESSY, CT
中科院分区：
文献类型：
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作者：
BOULTON, CL;MCCROHAN, CR;OSHAUGHNESSY, CT
本文研究了环腺苷酸激动剂8-（4-氯苯硫基）-腺苷3 '：5'环磷酸（CPT-cAMP）、二丁酰环腺苷酸（dbcAMP）和forskolin对离体大鼠脑片细胞外场电位的影响。CPT-cAMP和毛喉素产生了长期的增强癫痫样活动导致从浴介质中除去镁。DbcAMP没有明显的效果，除了在高浓度（1毫米），当它减少爆裂活动。场电位观察到的胼胝体在Mg 2+的存在下电刺激后，CPT-cAMP和dbcAMP增强，但毛喉素是没有效果。细胞内记录技术证明了短暂的兴奋性影响的dbcAMP。结果表明环磷酸腺苷在癫痫发作机制中的作用。
Effects of the cyclic AMP agonists 8-(4-chlorophenylthio)-adenosine 3': 5' cyclic monophosphate (CPT-cAMP), dibutyryl cyclic AMP (dbcAMP) and forskolin were studied on extracellular field potentials in rat neocortex slices in vitro. CPT-cAMP and forskolin produced a prolonged enhancement of epileptiform activity resulting from removal of Mg2+ from the bathing medium. DbcAMP had no apparent effect except at high concentrations (1 mM), when it reduced bursting activity. Field potentials observed following electrical stimulation of the corpus callosum in the presence of Mg2+ were enhanced by CPT-cAMP and dbcAMP; however forskolin was without effect. Intracellular recording techniques demonstrated a transient excitatory influence of dbcAMP. The results indicate a role for cyclic AMP in seizure mechanisms.