Involvement of LAT, Gads, and Grb2 in compartmentation of SLP-76 to the plasma membrane.
Involvement of LAT, Gads, and Grb2 in compartmentation of SLP-76 to the plasma membrane.
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DOI：
10.1084/jem.192.6.847
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发表时间：
2000-09-18
期刊：
影响因子：
--
通讯作者：
Kurosaki T
中科院分区：
文献类型：
--
作者：
Ishiai M;Kurosaki M;Inabe K;Chan AC;Sugamura K;Kurosaki T
B细胞连接蛋白（BLNK）和76 kD的含Src同源2结构域的白细胞蛋白（SLP-76）分别是B细胞受体（BCR）和T细胞受体功能所需的衔接蛋白。在这里，我们发现SLP-76的表达不能重建Zap-70+BLNK− B细胞中的BCR功能。这可能是由于抗原受体交联后SLP-76不能被募集到富含糖脂的微区（GEM）中。支持这一观点的是，当膜相关的SLP-76嵌合体被强制定位于GEM时，BCR功能恢复。此外，我们证明了向SLP-76添加用于活化T细胞的接头（LAT）和Shc下游的Grb 2相关衔接子（Gads）允许SLP-76被募集到GEM中，由此重建BCR功能。Gads功能能够被Grb 2的过表达所取代。与SLP-76相比，BLNK不需要Grb 2家族来招募GEM。因此，这些数据表明BLNK和SLP-76之间的功能重叠，同时强调了在靶向GEM时对额外衔接子分子的需求的差异。
B cell linker protein (BLNK) and Src homology 2 domain–containing leukocyte protein of 76 kD (SLP-76) are adaptor proteins required for B cell receptor (BCR) and T cell receptor function, respectively. Here, we show that expression of SLP-76 cannot reconstitute BCR function in Zap-70+BLNK− B cells. This could be attributable to inability of SLP-76 to be recruited into glycolipid-enriched microdomains (GEMs) after antigen receptor cross-linking. Supporting this idea, the BCR function was restored when a membrane-associated SLP-76 chimera was enforcedly localized to GEMs. Moreover, we demonstrate that addition of both linker for activation of T cells (LAT) and Grb2-related adaptor downstream of Shc (Gads) to SLP-76 allow SLP-76 to be recruited into GEMs, whereby the BCR function is reconstituted. The Gads function was able to be replaced by overexpression of Grb2. In contrast to SLP-76, BLNK did not require Grb2 families for its recruitment to GEMs. Hence, these data suggest a functional overlap between BLNK and SLP-76, while emphasizing the difference in requirement for additional adaptor molecules in their targeting to GEMs.
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影响因子：
32.4
作者：
Liou, J;Kiefer, F;Weiss, A
通讯作者：
Weiss, A
影响因子：
32.4
作者：
KONG, GH;BU, JY;CHAN, AC
通讯作者：
CHAN, AC
DOI：
10.1084/jem.189.8.1243
发表时间：
1999-04-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Law CL;Ewings MK;Chaudhary PM;Solow SA;Yun TJ;Marshall AJ;Hood L;Clark EA
通讯作者：
Clark EA
影响因子：
9.2
作者：
Liu, SK;Fang, N;McGlade, CJ
通讯作者：
McGlade, CJ
影响因子：
32.4
作者：
Fu, C;Turck, CW;Chan, AC
通讯作者：
Chan, AC