Bilaterian phylogeny based on analyses of a region of the sodium-potassium ATPase β-subunit gene
Bilaterian phylogeny based on analyses of a region of the sodium-potassium ATPase β-subunit gene
复制标题
DOI：
10.1007/s00239-003-2548-9
复制
发表时间：
2004-03-01
影响因子：
3.9
通讯作者：
Thollesson, M
中科院分区：
文献类型：
--
作者：
Anderson, FE;Córdoba, AJ;Thollesson, M
由于缺乏适合分子系统学家研究的缓慢进化的基因，对动物门内部和门之间深层关系的分子研究受到了阻碍。为了提供用于深层次后生动物系统发育研究的新数据，开发了引物来扩增来自代表多个门的 31 种两侧对称动物的核编码钠钾 ATP 酶基因的 α 亚基的 1.3 kb 区域。对这些序列进行最大简约性、最大似然性和贝叶斯分析（与从 GenBank 下载的 23 个分类群的 ATPase 序列相结合）产生一致树，证实了基于其他数据集（例如 18S 核糖体 RNA 基因）分析的最新发现。基于 ATP 酶的树支持多个进化支（包括冠轮动物门、蜕皮动物门、脊椎动物、软体动物、双壳纲、腹足纲、蛛形纲、六足纲、鞘翅目和双翅目）的单系，但不支持后口纲、节肢动物或纽虫纲的单系。参数引导测试拒绝节肢动物和纽虫的单系性，但无法拒绝后口动物的单系性。总体而言，钠钾 ATP 酶 α 亚基基因似乎对于后生动物系统发育的深入研究有用。
Molecular investigations of deep-level relationships within and among the animal phyla have been hampered by a lack of slowly evolving genes that are amenable to study by molecular systematists. To provide new data for use in deep-level metazoan phylogenetic studies, primers were developed to amplify a 1.3-kb region of the alpha subunit of the nuclear-encoded sodium-potassium ATPase gene from 31 bilaterians representing several phyla. Maximum parsimony, maximum likelihood, and Bayesian analyses of these sequences (combined with ATPase sequences for 23 taxa downloaded from GenBank) yield congruent trees that corroborate recent findings based on analyses of other data sets (e.g., the 18S ribosomal RNA gene). The ATPase-based trees support monophyly for several clades (including Lophotrochozoa, a form of Ecdysozoa, Vertebrata, Mollusca, Bivalvia, Gastropoda, Arachnida, Hexapoda, Coleoptera, and Diptera) but do not support monophyly for Deuterostomia, Arthropoda, or Nemertea. Parametric bootstrapping tests reject monophyly for Arthropoda and Nemertea but are unable to reject deuterostome monophyly. Overall, the sodium-potassium ATPase alpha-subunit gene appears to be useful for deep-level studies of metazoan phylogeny.