Symbiosis and insect diversification:: An ancient symbiont of sap-feeding insects from the bacterial phylum Bacteroidetes
Symbiosis and insect diversification:: An ancient symbiont of sap-feeding insects from the bacterial phylum Bacteroidetes
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DOI：
10.1128/aem.71.12.8802-8810.2005
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发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Gerardo, NM
中科院分区：
文献类型：
--
作者：
Moran, NA;Tran, P;Gerardo, NM
有几个昆虫群具有专性的、垂直传播的细菌共生体，为宿主提供限制饮食的营养。其中一些细菌被证明是从古代感染而来的。在这里，我们展示了一大群相关昆虫，包括蝉、叶蝉、树蝉、唾虫和稻飞虱，它们拥有不同的细菌共生体。这一新描述的共生体谱系属于拟杆菌门。对16S rRNA基因的分析表明，共生体的系统发育与目前已知的昆虫寄主的系统发育完全一致。这些结果支持这些昆虫的共同祖先在古代获得了共生体，将最初的感染追溯到至少2.6亿年前。如在一种唾虫(Cercopoidae)和一种神枪虫(叶蝉亚科)中所见，共生体具有非常大的细长形状的细胞，通常超过30微米长；原位杂交证实这些细胞与类杆菌门相对应。新的共生体命名为“Candidatus Sulcia muelleri”。
Several insect groups have obligate, vertically transmitted bacterial symbionts that provision hosts with nutrients that are limiting in the diet. Some of these bacteria have been shown to descend from ancient infections. Here we show that the large group of related insects including cicadas, leafhoppers, treehoppers, spittlebugs, and planthoppers host a distinct clade of bacterial symbionts. This newly described symbiont lineage belongs to the phylum Bacteroidetes. Analyses of 16S rRNA genes indicate that the symbiont phylogeny is completely congruent with the phylogeny of insect hosts as currently known. These results support the ancient acquisition of a symbiont by a shared ancestor of these insects, dating the original infection to at least 260 million years ago. As visualized in a species of spittlebug (Cercopoidea) and in a species of sharpshooter (Cicadellinae), the symbionts have extraordinarily large cells with an elongate shape, often more than 30 mu m in length; in situ hybridizations verify that these correspond to the phylum Bacteroidetes. "Candidatus Sulcia muelleri" is proposed as the name of the new symbiont.