N-omega-nitro-L-arginine methyl ester protects retinal neurons against N-methyl-D-aspartate-induced neurotoxicity in vivo
N-omega-nitro-L-arginine methyl ester protects retinal neurons against N-methyl-D-aspartate-induced neurotoxicity in vivo
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DOI：
10.1016/s0014-2999(97)83026-9
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发表时间：
1997-06-05
影响因子：
5
通讯作者：
Honda, Y
中科院分区：
文献类型：
--
作者：
Morizane, C;Adachi, K;Honda, Y
本实验研究了一氧化氮合酶竞争性抑制剂L抑制一氧化氮(NO)合成对N-甲基-D-天冬氨酸诱导的大鼠视网膜神经毒性的影响。注射后7天，单次玻璃体内注射NMDA损伤神经节细胞层和内网状层，而对其他视网膜层无影响。玻璃体内注射N-甲基-D-环庚烯-5，10-亚胺马来酸氢化物((5R，10S)-(+)-5-methyl-10，11-dihydro-5H-dibenzo[a，)可显著减轻N-甲基-D-天冬氨酸诱导的视网膜变性。玻璃体内注射L-NAME但不能阻止D-NAME所致的变性。L的保护作用可被L精氨酸拮抗。这些结果表明，NO在NMDA诱导的视网膜兴奋性毒性变性中起重要作用。
We investigated whether the inhibition of nitric oxide (NO) synthesis with N(o)mega-nitro-L-arginine methyl ester (L-NAME), a competitive inhibitor of NO synthase, affects N-methyl-D-aspartate (NMDA)-induced neurotoxicity in the rat retina in vivo. A single intravitreal injection of NMDA damaged the ganglion cell layer and the inner plexiform layer without affecting the other retinal layers 7 days after injection. Intravitreal injection of (5R,10S)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclo-hepten-5,10-imine hydrogen maleate (MK-801) with NMDA significantly reduced NMDA-induced degeneration of the retina. NMDA-induced degeneration was also prevented by intravitreal injection of L-NAME but not of D-NAME. The protective effect of L-NAME was antagonized by L-arginine. These results suggest that NO plays an important role in NMDA-induced excitotoxic degeneration in the retina.