HARVEY SARCOMA-VIRUS - SECOND MURINE TYPE-C SARCOMA-VIRUS WITH RAT GENETIC INFORMATION
HARVEY SARCOMA-VIRUS - SECOND MURINE TYPE-C SARCOMA-VIRUS WITH RAT GENETIC INFORMATION
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DOI：
10.1128/jvi.13.6.1211-1219.1974
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发表时间：
1974-01-01
影响因子：
5.4
通讯作者：
PARKS, WP
中科院分区：
文献类型：
--
作者：
SCOLNICK, EM;PARKS, WP
用RNA·[~ 3 H]DNA和[~ 3 H]RNA·DNA杂交技术分析了小鼠肉瘤病毒Harvey株的核酸序列。已经发现鼠肉瘤病毒的Harvey株具有至少两组核酸序列。一组序列包含在小鼠C型病毒的莫洛尼株中，另一组包含在含有大鼠C型病毒的内源性反应中合成的DNA转录物中。用大鼠C型病毒合成的DNA探针在哈维肉瘤病毒中检测到的核酸序列与在鼠肉瘤病毒Kirsten株中检测到的大鼠序列相关。结果支持的模型，Kirsten和Harvey肉瘤病毒都出现了通过小鼠C型病毒和大鼠细胞中的序列之间的重组或重配的过程中，并表明，转化成纤维细胞的信息可能包含在大鼠C型或细胞基因组。
The nucleic acid sequences found in the Harvey strain of murine sarcoma virus have been analyzed by RNA·[3H]DNA and [3H]RNA·DNA hybridization techniques. The Harvey strain of murine sarcoma virus has been found to possess at least two sets of nucleic acid sequences. One set of sequences is contained in the Moloney strain of mouse type-C virus, and the other set is contained in DNA transcripts synthesized in endogenous reactions containing rat type-C virus(es). The nucleic acid sequences that are detected in the Harvey sarcoma virus with the DNA probes synthesized from the rat type-C virus(es) are related to the rat sequences detected in the Kirsten strain of murine sarcoma virus. The results support the model that both Kirsten and Harvey sarcoma viruses arose through a process of recombination or reassortment between mouse type-C viruses and sequences in rat cells and suggest that the information for transformation of fibroblasts may be contained in the rat type-C or cellular genome.