Functional characterization of the PSII-LHC II supercomplex isolated by a direct method from spinach thylakoid membranes
Functional characterization of the PSII-LHC II supercomplex isolated by a direct method from spinach thylakoid membranes
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DOI：
10.1023/a:1006404302573
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发表时间：
2000-01-01
影响因子：
3.7
通讯作者：
Barber, J
中科院分区：
文献类型：
--
作者：
Eshaghi, S;Turcsányi, E;Barber, J
最近，报道了一种直接从类囊体膜中分离高纯度和活性的光系统II制剂的新方法，称为PS II-LHC II超复合物[Eshaghi等（1999）FEBS Lett 446：23-26]。除了反应中心的核心蛋白外，超复合物还含有放氧复合物的所有外在蛋白和一组叶绿素a/B结合蛋白。在本文中，这个孤立的超配合物的功能特性，其特征在于进一步使用EPR光谱，热释光，荧光弛豫动力学和闪光诱导氧产率测量。PS Ⅱ-LHC Ⅱ超复合物除了Q（A）和Q（B）外，还含有少量质体醌（PQ）。虽然分离的复合物不再是膜结合的，它保留了一个明确的PS II制剂的功能特性与Q（B）网站的一些修改除外。
Recently, a novel procedure to isolate a highly pure and active Photosystem II preparation directly from thylakoid membranes, referred to as PS II-LHC II supercomplex, was reported [Eshaghi et al. (1999) FEBS Lett 446: 23-26]. In addition to the reaction center core proteins, the supercomplex contains all the extrinsic proteins of the oxygen evolving complex and a set of chlorophyll a/b binding proteins. In this paper, the functional properties of this isolated supercomplex are further characterized by using EPR spectroscopy, thermoluminescence, fluorescence relaxation kinetics and flash induced oxygen yield measurements. The PS II-LHC II supercomplex contains, in addition to Q(A) and Q(B), a small pool of plastoquinone (PQ). Although the isolated complex is no longer membrane bound, it has preserved functional characteristics of a well defined PS II preparation with the exception of some modification of Q(B) sites.