The xenobiotic transporter ABCG2 plays a novel role in differentiation of trophoblast-like BeWo cells
The xenobiotic transporter ABCG2 plays a novel role in differentiation of trophoblast-like BeWo cells
复制标题
DOI：
10.1016/j.placenta.2006.12.003
复制
发表时间：
2007-04-01
期刊：
影响因子：
3.8
通讯作者：
Keelan, J. A.
中科院分区：
文献类型：
--
作者：
Evseenko, D. A.;Paxton, J. W.;Keelan, J. A.
滋养层细胞在细胞融合过程中经历质膜脂质不对称性的丧失，而没有进一步进展到凋亡的终末阶段。在此过程中提供细胞存活的抗凋亡机制的性质是未知的。使用BeWo细胞模型，我们探讨了异生物质/脂质转运蛋白ABCG 2在促进毛喉素诱导分化过程中的细胞存活中的作用。通过siRNA抑制ABCG 2表达导致磷脂酰丝氨酸外化显著增加，随后是神经酰胺的积累和细胞凋亡增加。ABCG 2沉默降低了合胞体形成标志物（β-hCG和HERV-W）的表达，但融合不受影响。这些研究结果表明，ABCG 2保护细胞在短暂的膜不稳定与细胞分化和融合，突出了一个新的，以前未认识到的作用，ABCG 2作为一个生存因子在胎盘合胞体的形成。(c)2007年由IFPA和Elsevier Ltd.出版
Trophoblast cells undergo loss of plasma membrane lipid asymmetry during cell fusion without further progression to terminal phases of apoptosis. The nature of the anti-apoptotic mechanisms providing cell survival during this process is unknown. Using a BeWo cell model, we explored the role of the xenobiotic/lipid transporter ABCG2 in promoting cell survival during forskolin-induced differentiation. Suppression of ABCG2 expression by siRNA led to a marked increase in phosphatidylserine externalisation followed by accumulation of ceramides and increased apoptosis. Expression of markers of syncytial formation (beta-hCG and HERV-W) was decreased by ABCG2 silencing, although fusion was unaffected. These findings suggest that ABCG2 protects cells during the period of transient membrane instability associated with cell differentiation and fusion, highlighting a novel, previously unrecognised role of ABCG2 as a survival factor during the formation of the placental syncytium. (c) 2007 Published by IFPA and Elsevier Ltd.