The antifungal activity of the terrestrial Streptomyces US80 strain is induced by heat-killed fungi
The antifungal activity of the terrestrial Streptomyces US80 strain is induced by heat-killed fungi
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DOI：
10.1002/biot.200700155
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发表时间：
2008-08-01
影响因子：
4.7
通讯作者：
Mellouli, Lotfi
中科院分区：
文献类型：
--
作者：
Fguira, Lilia Fourati-Ben;Smaoui, Slim;Mellouli, Lotfi
研究不同浓度葡萄糖作为碳源和镁作为化学添加剂对链霉菌产量的影响。三种抗真菌分子（伊鲁霉素、X-14952 B 和 17-羟基文丘里菌素 A）的 US80 菌株显示，对于葡萄糖和镁分别在 5 g/L 和 3.5 mM 时获得最高的抗真菌活性。产生最大抗真菌活性的环境因素是：生长温度30℃、pH 7、孵育时间72小时和搅拌速率200rpm。进一步提高这三种在农业领域具有真正潜在应用潜力的抗真菌化合物的产量，并探索从链霉菌中获得其他活性分子的可能性。 US80，我们研究了在培养基中添加热灭活真菌的效果。链霉菌培养物的生化、微生物学和光谱研究。与对照培养物相比，不存在（对照）和存在热灭活真菌细胞的 US80 菌株表明三种抗真菌分子的产量增加了 70%。
Study of the influence of different concentrations of glucose, as carbon source, and magnesium, as chemical additive, on production by the Streptomyces sp. US80 strain of the three antifungal molecules (irumamycin, X-14952 B and 17-hydroxy-venturicidin A) showed that the highest antifungal activity was obtained at 5 g/L and 3.5 mM for glucose and magnesium, respectively. Environmental factors for maximum antifungal activity production are: temperature of growth 30 C, pH 7, incubation time 72 h and agitation rate of 200 rpm. To further enhance the production of the three antifungal compounds, which possess a real potential application in the agriculture domain, and to explore the possibility of obtaining other active molecules from the Streptomyces sp. US80, we investigated the effect of the addition of heat-killed fungi to the culture media. Biochemical, microbiological and spectroscopic studies of the cultures of the Streptomyces sp. US80 strain in absence (control) and in presence of heat-killed fungus cells indicate an increase of 70% in the production of the three antifungal molecules, compared to the control culture.