Postischemic liver damage in rats: effect of some therapeutic interventions on survival rate.
Postischemic liver damage in rats: effect of some therapeutic interventions on survival rate.
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DOI：
10.1620/tjem.138.63
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发表时间：
1982-09
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影响因子：
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通讯作者：
H. Yamauchi;I. Baća;U. Mittmann;H. Geisen;M. Salzer
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文献类型：
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作者：
H. Yamauchi;I. Baća;U. Mittmann;H. Geisen;M. Salzer
本文旨在阐明常温下肝脏部分（2/3）缺血90分钟后死亡的原因，并检查胰高血糖素、生长抑素、胰岛素、泼尼松龙和口服多粘菌素B（PB）的作用。将部分缺血90分钟后24小时的动物分为两组，即外观正常的动物和濒死状态的动物。两组大鼠的血浆S-GOT、S-GPT、氨基酸、NH3或胰岛素水平或坏死肝细胞的形态学估计体积比均无显著差异。然而，濒死大鼠的血糖水平显著降低（31 +/- 28 mg/100 ml，n = 6），与外观正常的大鼠（149 +/- 19，n = 5）相比，鲎凝胶试验阳性发生率更高。动物的1天和1周存活率分别为42/62（69%）和32/61（53%）。胰高血糖素注射液（1.5 mg/kg，缺血后）可有效提高1天存活率（14/14），但未能提高1周存活率（11/14）。另一方面，生长抑素注射液（100微克/公斤，缺血后）或PB治疗（15毫克/公斤/天× 5-9，缺血前）成功地增加了1周的生存率（20/22 p小于0.01和17/17 p小于0.01，分别），虽然转氨酶水平或坏死体积比没有显着改善。可以看出，部分缺血后的濒死状态伴随着严重的低血糖休克，缺血后注射生长抑素或缺血前口服多粘菌素B消灭革兰氏阴性菌可防止低血糖休克的发生。
The aim of this paper is to elucidate the cause of death after 90 min of normothermic partial (2/3) ischemia of the liver and to examine the effects of glucagon, somatostatin, insulin, prednisolone and oral administration of polymyxin B (PB). The animals 24 hr after partial ischemia for 90 min were divided into two groups; namely, animals with normal appearance and those with moribund state. There were no significant differences in the plasma level of S-GOT, S-GPT, amino acids, NH3 or insulin, or in morphometrically estimated volume ratio of necrotic hepatocytes between the two groups of rats. The blood glucose level, however, was significantly decreased (31 +/- 28 mg/100 ml, n = 6) in the moribund rats with a higher incidence of positive Limulus gelation tests as compared with the rats with normal appearance (149 +/- 19, n = 5). The 1-day and 1-week survival rates of the animals were 42/62 (69%) and 32/61 (53%), respectively. A glucagon injection (1.5 mg/kg, after ischemia) was effective to elevate the 1-day survival rate (14/14), but failed to increase the 1-week survival rate (11/14). On the other hand, a somatostatin injection (100 micrograms/kg, after ischemia) or PB treatment (15 mg/kg/day x 5-9, before ischemia) succeeded to increase the 1-week survival rate (20/22 p less than 0.01 and 17/17 p less than 0.01, respectively), although no significant amelioration in transaminase levels or volume ratio of necrosis was demonstrated. It could be seen that a moribund state after partial ischemia was accompanied by severe hypoglycemic shock, and that the injection of somatostatin after ischemia or the annihilation of gram-negative bacteria by means of oral administration of polymyxin B before ischemia prevented the occurrence of the hypoglycemic shock.