Conjugating AIE-featured AuAg nanoclusters with highly luminescent carbon dots for improved visible-light-driven antibacterial activity
Conjugating AIE-featured AuAg nanoclusters with highly luminescent carbon dots for improved visible-light-driven antibacterial activity
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将具有 AIE 功能的 AuAg 纳米团簇与高发光碳点结合，以提高可见光驱动的抗菌活性
DOI：
10.1039/d2nr01550a
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Xun Yuan
中科院分区：
文献类型：
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作者：
Naiwei Liu;Yichun Wang;Ziping Wang;Qiuxia He;Yong Liu;Xinyue Dou;Zhengmao Yin;Yan Li;Haiguang Zhu;Xun Yuan
金属纳米簇(NCS)是一种新型抗菌剂，具有广谱抗菌活性，对细菌无耐药性，但因其被细菌消耗而快速失效。在这里，我们报道了一种基于共轭聚集诱导发射(AIE)的具有高发光碳点(Cd)的AuAg纳米CS的可见光驱动光动力学抗菌剂的设计。硫化镉与AuAgNCS的偶联不仅可以提高可见光的获得率，而且可以通过电荷/能量转移促进载流子的产生/分离，从而在可见光照射下产生丰富的活性氧物种(ROS)用于杀菌。结果表明，所制得的CDS@AuAgNCS对革兰氏阳性菌和革兰氏阴性菌均表现出良好的光动力抗菌活性，菌落形成单位减少了4-5个数量级，不同于传统的依赖自消耗杀菌的金属NC类似物。此外，研究还发现，CDS@AuAg NCS没有细胞毒性；ROS捕获实验表明，CDS@AuAg NCS光解产生的过氧化氢是主要的杀菌活性物种，占总抗菌效果的近48%。本研究为金属纳米光动力抗菌剂的设计提供了一种新的范式。
Metal nanoclusters (NCs) have emerged as novel antibacterial agents featuring broad-spectrum antibacterial activity without drug resistance for bacteria, but suffer from fast antibacterial invalidation due to their consumption by bacteria. Herein we report the design of a visible-light-driven photodynamic antibacterial agent based on conjugating aggregation-induced emission (AIE)-featured AuAg NCs with highly luminescent carbon dots (CDs). The conjugation of CDs with AuAg NCs could not only enhance the visible-light harvest, but also promote charge carrier generation/separation via charge/energy transfer, leading to the production of abundant reactive oxygen species (ROS) for bacterial killing under visible-light irradiation. Consequently, the as-obtained CDs@AuAg NCs display excellent photodynamic antibacterial activity against both Gram-positive and Gram-negative bacteria with 4-5 orders of magnitude reduction in colony forming units, which is different from the conventional metal NC-based analogue relying on self-consumption for bacterial killing. In addition, the CDs@AuAg NCs are found to be free of cytotoxicity; the ROS capture experiments indicate that the photoproduced H2O2 by CDs@AuAg NCs is the main active species for bacterial killing, accounting for nearly 48% of the total antibacterial efficacy. This study provides a paradigm for the design of metal NC-based photodynamic antibacterial agents for diverse bactericidal applications.