Identification of signature proteins that are distinctive of the Deinococcus-Thermus phylum
Identification of signature proteins that are distinctive of the Deinococcus-Thermus phylum
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DOI：
10.2436/20.1501.01.28
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发表时间：
2007-09-01
影响因子：
3.1
通讯作者：
Gupta, Radhey S.
中科院分区：
文献类型：
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作者：
Griffiths, Emma;Gupta, Radhey S.
热球菌门的成员，包括许多耐极端辐射的物种，以及一些嗜热动物，仅根据它们在16S rRNA和其他系统发育树中的分支模式被识别出来。目前还不知道这类物种有什么独特的生化或生理特征。为了鉴定该物种所独有的基因/蛋白，对耐辐射球菌基因组的每个开放阅读框（ORF）进行了系统的蛋白质基本局部比对工具（Blastp）搜索。这些研究确定了65种蛋白质，这些蛋白质只在所有三个测序的热球菌基因组中发现（即耐辐射球菌、地热球菌和嗜热细菌），而在其他细菌中没有发现。此外，这些研究还发现了206种蛋白质仅存在于两种Deinocococci物种中，399种蛋白质仅存在于D. radiodurans中。所鉴定的蛋白质代表了Deinococci - thermus组或Deinococci物种特有的遗传库，为其鉴定和表征提供了新的分子标记。大多数这些蛋白质的细胞功能尚不清楚，它们的研究应该证明有助于识别这些细菌群所独有的新的生化和生理特征，以及那些导致耐辐射的耐辐射球菌的特征。
The members of the Deinococcus-Thermus phylum, which include many species that are resistant to extreme radiation, as well as several thermophiles, have been recognized solely on the basis of their branching patterns in 16S rRNA and other phylogenetic trees. No biochemical or physiological characteristic is currently known that is unique to this group of species. To identify genes/proteins that are exclusive of this group of species, systematic protein basic local alignment tool (Blastp) searches were carried out on each open reading frame (ORF) in the genome of Deinococcus radiodurans. These studies identified 65 proteins that were only found in all three sequenced Deinococcus-Thermus genomes (viz. D. radiodurans, D. geothermalis and Thermus thermophilus), but not in any other bacteria. In addition, these studies also identified 206 proteins that are exclusively found in the two Deinocococci species, and 399 proteins that are unique to D. radiodurans. The identified proteins, which represent a genetic repertoire distinctive to the Deinococcus-Thermus group, or to Deinococci species, provide novel molecular markers for their identification and characterization. The cellular functions of most of these proteins are not known and their studies should prove useful in identifying novel biochemical and physiological characteristics that are exclusive of these groups of bacteria and also those responsible for the extreme radiation resistance of Deinococci.