First characterization of bacterial pathogen, Vibrio alginolyticus, for Porites andrewsi White syndrome in the South China Sea.
First characterization of bacterial pathogen, Vibrio alginolyticus, for Porites andrewsi White syndrome in the South China Sea.
复制标题
南海滨珊瑚病原菌溶藻弧菌的首次鉴定
DOI：
10.1371/journal.pone.0075425
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yongcan Z
中科院分区：
文献类型：
--
作者：
Zhenyu X;Shaowen K;Chaoqun H;Zhixiong Z;Shifeng W;Yongcan Z
White综合征是一种具有进行性组织丧失的硬化性珊瑚疾病，已知会导致世界主要海洋中珊瑚礁的生长抑制和道德增加，在过去的几十年里，这种疾病的发生经常被报道。2010年4月至2011年9月的调查发现，南中国海西沙群岛普遍存在白斑海豚综合征（PAWS）。然而，爪子的原因和病因尚不清楚。研究方法/主要发现：对P。祁连鱼亚群（QLY）中的andrewsi。结果表明，在排除无效重复的情况下，28 d后试验组与对照组之间差异显著（P≤0.05）。组织损失率为0.90 ~ 10.76 cm2 d-1，平均值为5.40±3.34 cm2 d-1（平均值±SD）。从南海西沙群岛QLY疾病爆发地点的PAWS珊瑚中分离出菌株，并将其纳入实验室感染试验，以满足Koch建立因果关系的假设。暴露于105 mL-1的细菌浓度后，受感染的菌落表现出与野外观察到的相似的迹象。采用系统发育16S rRNA基因分析、经典表型性状比较、生物学自动鉴定系统、MALDI- tof质谱法和MALDI Biotyper法鉴定2株致病菌株为溶藻弧菌。结论/意义这是V。藻酸解菌在南海被认为是一种致病菌。我们的研究结果表明，迫切需要开发灵敏的V检测方法。南中国海溶藻毒力菌株和由该和致病菌弧菌引起的珊瑚病的可靠诊断。
Background White syndrome, a term for scleractinian coral disease with progressive tissue loss, is known to cause depressed growth and increased morality of coral reefs in the major oceans around the world, and the occurrence of this disease has been frequently reported in the past few decades. Investigations during April to September in both 2010 and 2011 identified widespread Porites andrewsi White syndrome (PAWS) in Xisha Archipelago, South China Sea. However, the causes and etiology of PAWS have been unknown. Methodology/Principal Findings A transmission experiment was performed on P . andrewsi in the Qilianyu Subgroup (QLY). The results showed that there was a significant (P ≤ 0.05) difference between test and control groups after 28 days if the invalid replicates were excluded. Rates of tissue loss ranged from 0.90-10.76 cm2 d-1 with a mean of 5.40 ± 3.34 cm2 d-1 (mean ± SD). Bacterial strains were isolated from the PAWS corals at the disease outbreak sites in QLY of the Xisha Archipelago, South China Sea, and included in laboratory-based infection trials to satisfy Koch’s postulates for establishing causality. Following exposure to bacterial concentrations of 105 cells mL-1, the infected colonies exhibited similar signs to those observed in the field. Using phylogenetic 16S rRNA gene analysis, classical phenotypic trait comparison, Biolog automatic identification system, MALDI-TOF mass spectrometry and MALDI Biotyper method, two pathogenic strains were identified as Vibrio alginolyticus . Conclusion/Significance This is the first report of V . alginolyticus as a pathogenic agent of PAWS in the South China Sea. Our results point out an urgent need to develop sensitive detection methods for V . alginolyticus virulence strains and robust diagnostics for coral disease caused by this and Vibrio pathogenic bacterium in the South China Sea.