SEM Observation of Wet Biological Specimens Pretreated with Room-Temperature Ionic Liquid
SEM Observation of Wet Biological Specimens Pretreated with Room-Temperature Ionic Liquid
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DOI：
10.1002/cbic.201100476
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发表时间：
2011-11-25
期刊：
影响因子：
3.2
通讯作者：
Kuwabata, Susumu
中科院分区：
文献类型：
--
作者：
Tsuda, Tetsuya;Nemoto, Noriko;Kuwabata, Susumu
一种简便的扫描电镜预处理工艺：使用室温离子液体（RTILs）为生物样品的扫描电镜提供了一种有趣的方法。我们使用了一种新颖而简洁的预处理方法，排除了固定或Au溅射步骤。以这种方式处理的各种生物标本的精细和光滑纹理的SEM图像被观察到没有伪影。
A facile pretreatment process for SEM: The use of room temperature ionic liquids (RTILs) provides an interesting method for SEM of biological specimens. We used a novel and concise method of pretreatment, excluding fixation or Au sputtering steps. Fine and smooth-textured SEM images of a wide variety of biological specimens treated in this way were observed without artefacts.